Dextran sulfate prevents LTB4-induced permeability increase, but not neutrophil emigration, in the hamster cheek pouch.

Microvascular Research
S RosengrenK E Arfors

Abstract

Leukotriene B4 (LTB4) is known to induce neutrophil-dependent macromolecular leakage in the hamster cheek pouch. LTB4 was superfused over cheek pouches dissected for intravital microscopy; and leukocyte rolling and firm adhesion in venules, neutrophil emigration, and macromolecular permeability as leakage of fluorescent dextran was quantified. Dextran sulfate (MW 500,000; 17.5 mg/kg iv bolus), but not uncharged dextran, reduced the LTB4-induced venular leakage of macromolecules by 85%. Histamine-induced leakage, which is neutrophil independent, was left unaffected. Dextran sulfate had no effect on leukocyte adhesion in postcapillary venules induced by LTB4, nor on their subsequent emigration to the surrounding tissue. Dextran sulfate significantly inhibited leukocyte rolling during nonstimulated conditions along the walls of collecting venules, but not postcapillary venules. Consequently, neutrophil-induced macromolecular leakage and neutrophil emigration appear to be independent, separable events. As an explanation of the present results it is proposed that the dextran sulfate complexes neutrophil granule cationic proteins, thus preventing neutrophil-mediated permeability increase.

References

May 1, 1985·Microvascular Research·U NobisP Gaehtgens
Oct 1, 1968·The Journal of Experimental Medicine·N S Ranadive, C G Cochrane
Apr 1, 1984·The Journal of Clinical Investigation·V M VehaskariA M Robson
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Samuelsson
Jan 1, 1980·Microvascular Research·G W Schmid-SchönbeinS Chien

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Citations

Jan 1, 1997·Free Radical Biology & Medicine·G HotterJ Roselló-Catafau
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·U H von AndrianE C Butcher
Jun 1, 1995·The Journal of Experimental Medicine·M SubramaniamD D Wagner
Nov 1, 2011·Microvascular Research·Erik SvensjöJulio Scharfstein
Oct 5, 2013·Blood·Klaus Ley
Oct 8, 2016·Small GTPases·Lilian SchimmelJaap D van Buul
Dec 1, 1992·The Journal of Surgical Research·M RichardsonJ A Freischlag
Oct 9, 2001·Nature Medicine·K Ley
Oct 6, 2016·F1000Research·Debashree Goswami, Dietmar Vestweber
Jan 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·F E PollockB P Smith
Oct 4, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ellinor KenneLennart Lindbom

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