Dextransucrase from the mutant of Pediococcus pentosaceus (PPm) is more stable than the wild type.

3 Biotech
Damini KothariArun Goyal

Abstract

A comparative study on both wild type and mutant of Pediococcus pentosaceus for dextransucrase activity, its stability, dextran synthesizing activity, antibiotic sensitivity and carbohydrate utilization was performed. The wild type P. pentosaceus had specific activity of 0.58 U/mg whereas the mutant showed that of 1.0 U/mg with 72% enhancement. The antibiogram of 27 antibiotics tested against mutant showed significant differences with 9 antibiotics when compared to wild type. In carbohydrate fermentation profile, trehalose, galactose, maltose, lactose and fructose are metabolized by both the strains, but weakly in case of mutant. Stabilization of purified dextransucrase from wild type and mutant with various stabilizers was studied at 30 and 4 °C. Both enzymes were more stable at 4 °C. Among various stabilizers such as dextran (100 kDa, 10 μg/ml), glycerol (0.5%, v/v), PEG 8000 (10 μg/ml) and Tween 80 (0.5%, v/v), Tween 80 provided maximum stabilization at 4 and 30 °C. The mutant showed better stabilization than that of the wild type at both 30 and 4 °C. The loss of activity at 30 °C after 24 h in wild type and mutant in the presence of Tween 80 was only 34 and 32%, respectively, whereas the loss of activity in control of wild ...Continue Reading

References

Feb 1, 1985·European Journal of Clinical Microbiology·P C FuchsR N Jones
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·R L Sidebotham
May 11, 1999·FEMS Microbiology Reviews·L De Vuyst, B Degeest
Mar 27, 2001·Letters in Applied Microbiology·S M HoltK J Shin
May 15, 2003·International Journal of Food Microbiology·J K MugulaT Sørhaug
Aug 20, 2005·Journal of Applied Microbiology·M TiekingM G Gänzle
Jul 16, 2010·Food Microbiology·Luca Settanni, Giancarlo Moschetti
Aug 7, 2010·Food Microbiology·Svetoslav Dimitrov TodorovDjamel Drider
Oct 1, 2010·Indian Journal of Microbiology·Ravi Kiran PuramaArun Goyal

❮ Previous
Next ❯

Citations

Apr 18, 2015·International Journal of Biological Macromolecules·Rashida Rahmat ZohraShah Ali Ul Qader
Jun 1, 2017·Probiotics and Antimicrobial Proteins·Pamela Oliveira de Souza de AzevedoRicardo Pinheiro de Souza Oliveira

❮ Previous
Next ❯

Datasets Mentioned

BETA
EU569832

Methods Mentioned

BETA
electrophoresis

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.