DH1, a LOB domain-like protein required for glume formation in rice

Plant Molecular Biology
A LiX Pan

Abstract

T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% florets on the dh1 panicle defected in forming normal palea and lemma. Instead, they formed degenerative velum-like or filamentous organs accompanying with the lack of lodicules, stamens and pistils at different degree. A 361 bp of genomic sequence flanking the T-DNA isolated using TAIL-PCR (Thermal asymmetric interlaced PCR) co-segregated with the mutation phenotype. Results of blastn and gene prediction revealed the T-DNA inserted into the promoter region of a function-predicted gene at 283 bp upstream of its transcription start site (TSS). The predicted gene encoded a LOB (Lateral Organ Boundaries) domain-like protein. RT-PCR analyses indicated the transcription level of target candidate gene, DH1, decreased significantly in dh1 mutant. RNAi aimed at DH1 in wild type plants could partially result in the mutation phenotype of dh1. DH1 could also rescue the mutation phenotype in the complement experiment. The resul...Continue Reading

References

Sep 1, 1990·Genes & Development·R Carpenter, E S Coen
Aug 1, 1990·The Plant Cell·V F Irish, I M Sussex
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
Nov 1, 1995·The Plant Cell·L ColomboA J van Tunen
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Cook
Jun 26, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Y ShinozukaT Sasaki
Jul 8, 1999·Molecular & General Genetics : MGG·J Serrano-CartagenaJ L Micol
Mar 9, 2000·Trends in Plant Science·J L Bowman, Y Eshed
Apr 25, 2000·Cell·H Ma, C dePamphilis
Apr 28, 2000·Nature·G Theissen
Feb 13, 2001·Current Opinion in Plant Biology·S E Clark
Feb 13, 2001·Current Opinion in Plant Biology·G Theissen
Feb 24, 2001·Nature·G Theissen, H Saedler
Mar 21, 2001·Nature Reviews. Genetics·M Ng, M F Yanofsky
Jun 1, 2002·Nature Genetics·Sarah Hake, Naomi Ori
Jun 18, 2002·Plant Physiology·Bin ShuaiPatricia S Springer
Dec 31, 2002·Development·Nobuhiro NagasawaYasuo Nagato
Feb 13, 2003·Journal of Plant Research·Shinobu Takada, Masao Tasaka
Feb 28, 2003·The EMBO Journal·Emma KeckEnrico Coen
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Changyin WuQifa Zhang
Nov 15, 2003·Molecular Phylogenetics and Evolution·Annette Becker, Günter Theissen
Jan 21, 2004·Current Opinion in Plant Biology·Bruce Veit
Mar 9, 2004·Current Opinion in Plant Biology·Michel Delseny
Jun 18, 2005·The Plant Journal : for Cell and Molecular Biology·Hongjia LiuPing Wu

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Citations

May 22, 2013·Plant Cell Reports·Xiangjin WeiPeisong Hu
May 26, 2010·Functional & Integrative Genomics·SuiKang WangYanHua Qi
Dec 29, 2011·Plant Molecular Biology·Xiaojiao LiChuanqing Sun
Mar 17, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lilan HongZhukuan Cheng
Sep 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qianwen Sun, Dao-Xiu Zhou
Sep 15, 2011·Journal of Experimental Botany·Hitoshi Yoshida, Yasuo Nagato
Jul 12, 2013·Plant Physiology·Nils MuthreichFrank Hochholdinger
Jun 10, 2015·Journal of Plant Physiology·Inmaculada Yruela
Aug 25, 2015·Plant, Cell & Environment·Shuen-Fang LoSu-May Yu
Oct 18, 2012·Plant Signaling & Behavior·Amanda MangeonPatricia S Springer
Feb 13, 2019·Journal of Experimental Botany·Grace L ChongloiUsha Vijayraghavan
Apr 1, 2020·International Journal of Molecular Sciences·Yuwen ZhangXiangyuan Wan

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