Di- and oligosaccharide substrate specificities and subsite binding energies of pig intestinal glucoamylase-maltase

Archives of Biochemistry and Biophysics
S Günther, H Heymann

Abstract

The substrate specificity of pig intestinal glucoamylase-maltase was investigated. The alpha-1, beta-2-glycosidic bond of the disaccharide sucrose was not hydrolyzed. Various substrates with alpha-1,4-glycosidic bonds (maltose, maltooligosaccharides) were hydrolyzed with high maximal reaction velocities. Reduction lowered the rate of hydrolysis drastically: k'0 decreases from 75 s-1 for maltose to 3 s-1 for maltitol while the K(m) value increases from 4.2 to 50 mM. Leucrose with alpha-1,5-glycosidic bond was hydrolyzed with a k'0 value of 8 s-1 and a K(m) value of 74 mM. Disaccharides with alpha-1,6-glycosidic bonds were hydrolyzed with extremely low rates: for isomaltose and isomaltulose k'0 values of 5 and 3 s-1, respectively, and K(m) values of 90 and 42 mM, respectively, were observed. Again reduction lowers the k'0 values: The corresponding disaccharide alcohols alpha-D-glucopyranosyl-1,6-sorbitol and alpha-D-glucopyranosyl-1,6-mannitol, like isomaltooligosaccharides, were not hydrolyzed. Regarding the conformation of sucrose, leucrose, and maltose previously determined by molecular dynamics simulations, a reasonable explanation for the different rates of hydrolysis could be postulated. Based on the enzyme kinetic paramete...Continue Reading

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Citations

Dec 29, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Saleh A MohamedTarek M Mohamed
Nov 14, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Saleh A MohamedHala A Salah
Jun 27, 2007·Journal of Pediatric Gastroenterology and Nutrition·Roberto Quezada-CalvilloBuford L Nichols
Jun 15, 2010·Medicine and Science in Sports and Exercise·Daniel J WestRichard M Bracken
Nov 1, 2012·Journal of Pediatric Gastroenterology and Nutrition·Amy Hui-Mei LinBuford L Nichols
Mar 22, 2008·The Journal of Nutrition·Roberto Quezada-CalvilloBuford L Nichols
Apr 24, 2007·The Journal of Nutrition·Juul AchtenAsker E Jeukendrup
Jun 23, 2007·The Journal of Nutrition·Roberto Quezada-CalvilloBuford L Nichols
Dec 9, 2000·The Journal of Nutrition·M J GnothS Rudloff

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