Di (2-Ethylhexyl) Phthalate and Its Role in Developing Cholestasis: An In Vitro Study on Different Liver Cell Types

Journal of Pediatric Gastroenterology and Nutrition
Haristi GaitantziUlrike Subotic

Abstract

Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in many polyvinylchloride medical devices and is washed out easily. Thereby critically ill infants can become exposed to DEHP concentrations significantly exceeding the recommended threshold. We suspect DEHP to play an important role in the development of intestinal failure-associated liver disease. The aim of this study was therefore to determine the direct influence of DEHP on different liver cell types. HepG2, human upcyte hepatocytes, primary murine hepatocytes, LX-2, human upcyte hepatic stellate cells, and liver organoids were cultured with DEHP (0.5-500 μmol/L) and parameters including cytotoxicity, cell-cell interactions, and expression of metabolizing enzymes were investigated. DEHP modulated the expression of xenobiotic metabolizing enzymes, reduced the formation of bile canaliculi and cell polarity, and inhibited Cyp-activity in hepatocytes. DEHP had a toxic effect on LX-2 and induced the fibrogenic activation of hepatic stellate cells. The mode of action of DEHP was different in monolayer cultures compared to 3D-liver organoids, which were more sensitive to DEHP. This study suggests that DEHP modulates expression and activity of drug-detoxifying liver enzymes i...Continue Reading

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S L FriedmanD M Bissell
Oct 1, 1993·Journal of Pediatric Surgery·R L MossJ G Raffensperger
Dec 31, 1997·Microscopy Research and Technique·W J Gallin
Sep 10, 2002·JPEN. Journal of Parenteral and Enteral Nutrition·Steffan LoffManuela Langbein
Apr 30, 2004·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·M Pinzani, K Rombouts
Sep 28, 2004·Journal of Pediatric Gastroenterology and Nutrition·Steffan LoffJ Brade
Feb 11, 2005·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Sandrine Bagel-BoithiasJean Chopineau
Apr 7, 2005·Environmental Health Perspectives·Russ HauserAntonia M Calafat
Apr 4, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Ma Concepción Gutiérrez-Ruiz, Luis Enrique Gómez-Quiroz
Mar 12, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Beth W CooperAndrew D Wallace
Aug 27, 2011·JPEN. Journal of Parenteral and Enteral Nutrition·Sandrine BagelValérie Sautou
Dec 27, 2011·Mutation Research·Ivan Rusyn, J Christopher Corton
May 30, 2013·Journal of Paediatrics and Child Health·Céline J FischerJean-François Tolsa
Oct 31, 2014·Journal of Perinatology : Official Journal of the California Perinatal Association·E B Mallow, M A Fox
Oct 22, 2015·PloS One·Sarada Devi RamachandranKatja Breitkopf-Heinlein
May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laia TolosaRamiro Jover
Jul 5, 2016·Journal of Developmental Origins of Health and Disease·J D Bowman, M Choudhury
Mar 25, 2017·Gut·Katja Breitkopf-HeinleinPeter Ten Dijke

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Citations

May 31, 2020·International Journal of Environmental Research and Public Health·Chun-Ya LeeYi-Jen Liao
Dec 29, 2020·Expert Opinion on Drug Safety·Jordan D SecorKathleen M Gura
Apr 6, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eva GijbelsMathieu Vinken
Jun 3, 2021·International Journal of Molecular Sciences·Raquel CanoValmore Bermúdez
Jun 28, 2019·Chemical Research in Toxicology·Vânia Vilas-BoasMathieu Vinken

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