Di-(2-ethylhexyl)-phthalate induces oxidative stress in human endometrial stromal cells in vitro

Molecular and Cellular Endocrinology
Yeon Jean ChoMyoungseok Han

Abstract

Di-(2-ethylhexyl)-phthalate (DEHP) accumulates in the environment, and its exposure is possibly associated with endocrine-related disease in women of reproductive age. The effects of DEHP on human endometrial cells are unknown. We treated human endometrial stromal cells with 10, 100, and 1000 pmol of DEHP and measured reactive oxygen species (ROS) generation, expression levels of antioxidant enzymes, alteration of MAPK/NF-κB signaling and hormonal receptors. DEHP increased reactive oxygen species (ROS) generation and decreased expression of superoxide dismutase (SOD), glutathione peroxidase (GPX), heme oxygenase (HO), and catalase (CAT). By DEHP exposure, p-ERK/p-p38 and NF-κB mediated transcription was increased. Additionally, DEHP induced estrogen receptor-α (ER-α) expression in a dose-dependent manner. This study shows the need for future mechanistic studies of oxidative stress, MAPK/NF-κB signaling, and ER-α as molecular mediators of DEHP-associated endometrial stromal cell alterations, which may be associated with the development of endocrine-related disease such as endometriosis.

References

Aug 1, 1993·Molecular and Cellular Endocrinology·A AriciM L Casey
Jul 3, 2002·Nature Reviews. Cancer·Igor Vivanco, Charles L Sawyers
Oct 23, 2003·Clinical Science·Masato IshizukaHironobu Sasano
Feb 16, 2005·Annual Review of Physiology·Sylvia C HewittKenneth S Korach
Jun 21, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Po-Len LiuYu Ru Kou
Jul 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Giuseppe Latini
Apr 28, 2006·BJOG : an International Journal of Obstetrics and Gynaecology·B S ReddyN V V S S Raman
Jun 23, 2007·Human Reproduction Update·S SimoensT D'Hooghe
Sep 25, 2007·International Journal of Hygiene and Environmental Health·Ursel HeudorfJürgen Angerer
Oct 22, 2008·Fertility and Sterility·D Andrew CrainLouis J Guillette
Jan 16, 2009·The New England Journal of Medicine·Serdar E Bulun
Jun 6, 2009·The American Journal of Pathology·Charlotte NgôFrédéric Batteux
Oct 9, 2009·The Science of the Total Environment·Hiroaki ItohShoichiro Tsugane
Feb 26, 2010·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Jan L LycheJanneche U Skaare
May 25, 2010·Fertility and Sterility·Young-Hoon KimByung Moon Kang
Jul 14, 2012·Archives of Gynecology and Obstetrics·Luiz Fernando Pina CarvalhoMauricio S Abrão
Sep 4, 2012·Fertility and Sterility·Sérgio Reis SoaresAntonio Pellicer
Oct 11, 2012·Journal of Hazardous Materials·Rui LiJunlin He
Jan 31, 2013·Toxicology and Applied Pharmacology·Lauren M TetzRita Loch-Caruso
Feb 15, 2013·Critical Reviews in Toxicology·Vanessa R KayWarren G Foster
Jul 31, 2013·Environmental Research·Kristen UpsonVictoria L Holt

❮ Previous
Next ❯

Citations

Jan 8, 2016·Human Reproduction Update·Brett D McKinnonMichael D Mueller
Feb 18, 2017·International Journal of Environmental Research and Public Health·I-Jen Wang, Wilfried J J Karmaus
Jan 15, 2019·Reproductive Biology and Endocrinology : RB&E·Justin RashtianZaher Merhi
Mar 26, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Xue WenYuanzhen Zhang
Jan 24, 2020·Obstetrics & Gynecology Science·Yeon Jean ChoHyun Young Kwon
May 16, 2020·Balsaeng'gwa saengsig·Yong-Pil Cheon
Aug 1, 2020·Balsaeng'gwa saengsig·Ju Hee Kim, Sung Hoon Kim
Sep 19, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chen-Yuan ChiuRong-Sen Yang
Apr 24, 2018·BioMed Research International·Sai Sandeep Singh Rowdhwal, Jiaxiang Chen
Jul 25, 2017·Environmental Science and Pollution Research International·Bingli LeiWei Peng
May 6, 2019·Reproductive Toxicology·Julia VarshavskyTracey J Woodruff
Apr 14, 2021·Environmental Science and Pollution Research International·Nuran Cikcikoglu YildirimOmer Samasas
Apr 15, 2021·Reproductive Medicine and Biology·Ya-Ching Chou, Chii-Ruey Tzeng
May 24, 2021·Environmental Science and Pollution Research International·Hamidreza Mohammadi, Sorour Ashari
Jun 11, 2021·Analytical Methods : Advancing Methods and Applications·Bukem TanorenMehmet Burcin Unlu
Jun 17, 2021·Journal of Environmental Sciences (China)·Jingjing LiYunhui Li
Jul 7, 2021·Journal of Assisted Reproduction and Genetics·Alessandro ConfortiCarlo Alviggi

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.