Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition against Gastric Cell via ROS-Mediated Apoptosis and Autophagy

Oxidative Medicine and Cellular Longevity
Xingshuang GuoChangzheng Li

Abstract

Diversified biological activities of dithiocarbamates have attracted widespread attention; improving their feature or exploring their potent action of mechanism is a hot topic in medicinal research. Herein, we presented a study on synthesis and investigation of a novel dithiocarbamate, DpdtbA (di-2-pyridylhydrazone dithiocarbamate butyric acid ester), on antitumor activity. The growth inhibition assay revealed that DpdtbA had important antitumor activity for gastric cancer (GC) cell lines (IC50 = 4.2 ± 0.52 μM for SGC-7901, 3.80 ± 0.40 μM for MGC-803). The next study indicated that growth inhibition is involved in ROS generation in mechanism; accordingly, the changes in mitochondrial membrane permeability, apoptotic genes, cytochrome c, bax, and bcl-2 were observed, implying that the growth inhibition of DpdtbA is involved in ROS-mediated apoptosis. On the other hand, the upregulated p53 upon DpdtbA treatment implied that p53 could also mediate the apoptosis. Yet the excess generation of ROS induced by DpdtbA led to cathepsin D translocation and increase of autophagic vacuoles and LC3-II, demonstrating that autophagy was also a contributor to growth inhibition. Further investigation showed that DpdtbA could induce cell cycle ar...Continue Reading

References

Aug 1, 1995·European Journal of Biochemistry·M T Elskens, M J Penninckx
Aug 1, 1995·Biological Trace Element Research·G VivoliE Caselgrandi
Dec 31, 1997·Biochimica Et Biophysica Acta·A M KomarovW B Weglicki
Jan 23, 1998·Chemical Research in Toxicology·C S NobelA F Slater
Jun 25, 1999·Free Radical Biology & Medicine·D MoelleringV M Darley-Usmar
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K KågedalK Ollinger
Sep 11, 2001·Cell Death and Differentiation·M Leist, M Jäättelä
Dec 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hamdiye ErdalStig Linder
May 18, 2005·Molecular Cancer Therapeutics·Lori Emert-SedlakDaniel E Johnson
Oct 29, 2008·Oncogene·P Boya, G Kroemer
Aug 14, 2009·Nature·Kevin J WaldronNigel J Robinson
Jan 16, 2010·Apoptosis : an International Journal on Programmed Cell Death·Ann-Charlotte JohanssonKarin Ollinger
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Mar 27, 2010·Current Pharmaceutical Design·Michael FrezzaQ Ping Dou
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Yongqiang ChenSpencer B Gibson
May 6, 2010·JAMA : the Journal of the American Medical Association·Xavier PaolettiMarc Buyse
Feb 4, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Satya S SadhuTeresa Seefeldt
Jul 24, 2012·Free Radical Biology & Medicine·Lin LiSpencer B Gibson
Jul 28, 2013·Nutrients·Jessica L HeathDaniel S Wechsler
Apr 1, 2015·Physiological Reports·Gamini Siriwardana, Paul A Seligman
May 30, 2015·Expert Opinion on Emerging Drugs·Bruno VincenziGiuseppe Tonini
Sep 19, 2015·Computational and Structural Biotechnology Journal·Xiandong LinBin Zhang
Nov 26, 2015·Annals of the New York Academy of Sciences·Ana Serrano-Puebla, Patricia Boya
Dec 20, 2015·Bioorganic & Medicinal Chemistry Letters·Vincent CorcéDavid Deniaud
Jan 20, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yi-Chao ZhengHong-Min Liu
Nov 27, 2016·Journal of Experimental & Clinical Cancer Research : CR·Wang LiuXiongwen Zhang
Nov 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martina ChripkovaJan Mojzis

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
flow cytometry
Assay
protein assay

Software Mentioned

CellQuest

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.