Di-Cysteine S,S-Tetrazine: A Potential Ultra-fast Photochemical Trigger to Explore the Early Events of Peptide/Protein Folding.

Journal of Photochemistry and Photobiology. A, Chemistry
Matthew J TuckerRobin M Hochstrasser

Abstract

The tetrazine chromophore holds promise as an effective photochemical trigger to achieve structural release, directed at the determination of peptide/protein motions that occur early in the folding processes. The photochemistry of 3,6-di-cysteine-S,S-tetrazines was examined by femtosecond IR transient absorption spectroscopy. Excitation of the tetrazine chromophore by visible and near UV light in the end yields chemically inert, structurally unobtrusive photoproducts that are not expected to interfere with the conformational dynamics of peptides and proteins. Dicysteine S,S-tetrazine is suggested to undergo photocleavage via a photochemical pathway different than the parent molecule s-tetrazine, based on kinetic measurements that reveal a stepwise reaction pathway of photofragmentation, whereby the initial ring cleavage event occurs prior to the formation of the SCN groups.

References

Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·D T LeesonR B Dyer
Sep 8, 2000·Physical Review Letters·G HummerS Garde
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Yen HuangFeng Gai
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sebastian SpörleinJosef Wachtveitl
May 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jens BredenbeckPeter Hamm
Apr 23, 2004·Current Opinion in Structural Biology·Jan KubelkaWilliam A Eaton
Jun 7, 2006·Photochemistry and Photobiology·Michael C PirrungJohn D Simon
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Janne A IhalainenPeter Hamm
Sep 4, 2007·The Journal of Physical Chemistry. B·Christoph KolanoPeter Hamm
Nov 13, 2007·Physical Review Letters·Stefan AuerMichele Vendruscolo
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Janne A IhalainenPeter Hamm
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jan KubelkaWilliam A Eaton
Apr 15, 2010·Angewandte Chemie·Matthew J TuckerRobin M Hochstrasser
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gregory R Bowman, Vijay S Pande
Oct 27, 2010·Journal of Molecular Biology·Stefano PianaDavid E Shaw
Feb 8, 2011·Nature Physics·Peter L FreddolinoKlaus Schulten
May 5, 2011·Biophysical Journal·Stefano PianaDavid E Shaw
May 17, 2011·The Journal of Physical Chemistry. B·Arnaldo L SerranoFeng Gai

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Citations

Oct 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Matthew J TuckerRobin M Hochstrasser
May 27, 2015·The Analyst·Amanda L Le SueurMegan C Thielges
Nov 7, 2018·Angewandte Chemie·Chi ZhangBradley L Pentelute
Jun 9, 2016·Angewandte Chemie·David E ChavezLauren Mitchell
Oct 6, 2020·Research : a Science Partner Journal·Changping WangYiyun Cheng
Jan 2, 2021·Chemical Science·Axel LoredoHan Xiao
Aug 17, 2020·Chemical Reviews·Xiang LiHong-Gang Hu
Dec 7, 2013·Analytical Chemistry·Lars KonermannModupeola A Sowole
May 8, 2015·The Journal of Physical Chemistry. a·Margo T GreenfieldR Jason Scharff

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