Di(2-ethylhexyl) phthalate inhibits B cell proliferation and reduces the abundance of IgM-secreting cells in cultured immune tissues of the rainbow trout

Fish & Shellfish Immunology
Kelly MartinsPatty Zwollo

Abstract

Plasticizer di(2-ethylhexyl) phthalate (DEHP) and its active metabolite MEHP have important immunotoxic effects in mammalian species, including inhibition of cell proliferation, inflammation inhibition, lowering of the antibody response, and apoptosis. Virtually nothing is known about the potential detrimental effects of DEHP/MEHP on the teleost immune system, although phthalates are a likely threat to fish health. Here we investigated whether short-term in vitro DEHP exposure would affect B lineage cells in the rainbow trout, using cultured immune tissues. Cell culture conditions, evidence of cellular incorporation of DEHP, and possible effects of DEHP on immune genes were first established using the mouse pre-B cell line PD31 and data confirmed a dose-dependent cellular uptake of DEHP using liquid chromatography-coupled ion trap mass spectrometry. Effects of in vitro DEHP exposure on trout B cell proliferation were tested by flow cytometry. Significant, dose-dependent inhibition was evident in both anterior and posterior kidney cultures after 24 h exposure to ≥4 μM DEHP. DEHP-induced cell death was not significant for the range of DEHP tested. Further, the abundance of IgM-secreting plasmablasts and plasma cells was significa...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 18, 2002·Toxicology Letters·Humitoshi SakazakiKatsuhiko Nakamuro
Oct 19, 2005·Biochimica Et Biophysica Acta·Carla Batista-PintoAlexandre Lobo-da-Cunha
Feb 10, 2006·International Journal of Andrology·Ted Schettler
Feb 24, 2006·Nature Reviews. Immunology·Christopher K Glass, Sumito Ogawa
Jul 13, 2006·Risk Analysis : an Official Publication of the Society for Risk Analysis·Matthias WormuthKonrad Hungerbühler
Apr 3, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jennifer J SchlezingerDavid H Sherr
Jul 12, 2008·Developmental and Comparative Immunology·Patty ZwolloJuliann Gumulak-Smith
Nov 20, 2008·Cancer Immunology, Immunotherapy : CII·Tatiana M Garcia-BatesRichard P Phipps
Dec 17, 2008·Experimental Cell Research·Shinu A John, Lee Ann Garrett-Sinha
Aug 14, 2010·Developmental and Comparative Immunology·Patty ZwolloMaggie Barr
Jan 22, 2011·Developmental and Comparative Immunology·Patty Zwollo
Feb 26, 2013·Developmental and Comparative Immunology·Jonathan SchoutenPatty Zwollo
Jun 26, 2013·Developmental and Comparative Immunology·Elizabeth MacMurrayPatty Zwollo
Jul 25, 2014·Environmental Geochemistry and Health·Krishna Kumar SelvarajBabu Rajendran Ramaswamy

❮ Previous
Next ❯

Citations

May 15, 2016·Archives of Environmental Contamination and Toxicology·Kelly MartinsPatty Zwollo
Apr 21, 2017·Critical Reviews in Toxicology·Kristina RehbergerLisa Baumann
Feb 24, 2018·Scientific Reports·Kristina SchaedlichJana Ernst
Feb 7, 2021·The Science of the Total Environment·Ying ZhangYang Yang
Mar 20, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Roya PournejatiHamid Reza Karbalaei-Heidari
Mar 21, 2021·The Science of the Total Environment·Abhijit MallikBinaya Bhusan Nayak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.