Diabetes Alters pH Control in Rat Retina

Investigative Ophthalmology & Visual Science
Andrey V DmitrievRobert A Linsenmeier

Abstract

The purpose of this study was to determine whether the ability of the rat retina to control its pH is affected by diabetes. Double-barreled H+-selective microelectrodes were used to measure extracellular [H+] in the dark-adapted retina of intact control and diabetic Long-Evans rats 1 to 6 months after intraperitoneal injection of vehicle or streptozotocin, respectively. Two manipulations-increasing of blood glucose and intravenous injection of the carbonic anhydrase blocker dorzolamide (DZM)-were used to examine their effects on retinal pH regulation. An increase of retinal acidity was correlated with the diabetes-related increase in blood glucose, but only between 1 and 3 months of diabetes, not earlier or later. Adding intravenous glucose had no noticeable effect on the retinal acidity of control animals. In contrast, similar injections of glucose in diabetic rats significantly increased the acidity of the retina. Again, the largest increase of retinal acidity due to artificially elevated blood glucose was observed at 1 to 3 months of diabetes. Suppression of carbonic anhydrase by DZM dramatically increased the retinal acidity in both control and diabetic retinas to a similar degree. However, in controls, the strongest effect...Continue Reading

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Jun 6, 2016·Experimental Eye Research·Andrey V DmitrievRobert A Linsenmeier
Jan 23, 2017·Progress in Retinal and Eye Research·Robert A Linsenmeier, Hao F Zhang
Apr 12, 2018·Current Eye Research·Alyssa DreffsRobert A Linsenmeier

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Citations

Feb 13, 2020·Investigative Ophthalmology & Visual Science·Mirella Telles Salgueiro BarboniJan Kremers
Dec 29, 2020·Heliyon·Robert A LinsenmeierAndrey V Dmitriev
Apr 14, 2020·Mitochondrion·Gabriela Ramírez-PérezRocío Salceda

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