Diabetes Alters the Involvement of Myofibroblasts During Periodontal Wound Healing

Oral Diseases
Ignacio RetamalP C Smith

Abstract

Myofibroblasts constitute a specific cell phenotype involved in connective tissue healing. Diabetes alters the wound healing response. However, it is not clear whether diabetes modifies the involvement of myofibroblasts in periodontal wounds. Type I diabetes was induced in rats through streptozotocin injection and periodontal wounds were performed. Wound healing was evaluated histologically at 2, 5, 7 and 15 days by measuring epithelial migration, neutrophil infiltration, collagen and biofilm formation. Distribution of myofibroblasts was evaluated through immunofluorescence for α-Smooth Muscle Actin. Data analyses were performed using the Shapiro-Wilk, ANOVA or Kruskal-Wallis tests. Diabetic wounds were characterized by delayed epithelial closure, increased neutrophil infiltration, biofilm formation and reduced collagen formation. Quantification of the myofibroblasts showed a significant reduction at 5 and 7 days in wounds of diabetic rats and an increase at 15 days when compared to wounds of non-diabetic rats. Diabetic wound healing was associated with decreased epithelial and connective tissue healing, increased levels of inflammation and biofilm formation. Myofibroblast differentiation was delayed in diabetic periodontal wou...Continue Reading

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Jun 23, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rou WanRobert D Galiano

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