Diabetes-associated microbiota in fa/fa rats is modified by Roux-en-Y gastric bypass

The ISME Journal
Tulika AroraFredrik Bäckhed

Abstract

Roux-en-Y gastric bypass (RYGB) and duodenal jejunal bypass (DJB), two different forms of bariatric surgery, are associated with improved glucose tolerance, but it is not clear whether the gut microbiota contributes to this effect. Here we used fa/fa rats as a model of impaired glucose tolerance to investigate whether (i) the microbiota varies between fa/fa and nondiabetic fa/+ rats; (ii) the microbiota of fa/fa rats is affected by RYGB and/or DJB; and (iii) surgically induced microbiota alterations contribute to glucose metabolism. We observed a profound expansion of Firmicutes (specifically, Lactobacillus animalis and Lactobacillus reuteri) in the small intestine of diabetic fa/fa compared with nondiabetic fa/+ rats. RYGB-, but not DJB-, treated fa/fa rats exhibited greater microbiota diversity in the ileum and lower L. animalis and L. reuteri abundance compared with sham-operated fa/fa rats in all intestinal segments, and their microbiota composition resembled that of unoperated fa/+ rats. To investigate the functional role of RYGB-associated microbiota alterations, we transferred microbiota from sham- and RYGB-treated fa/fa rats to germ-free mice. The metabolic phenotype of RYGB-treated rats was not transferred by the trans...Continue Reading

References

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Citations

Apr 27, 2019·Seminars in Immunopathology·Jean DebédatKarine Clément
Dec 6, 2019·Current Diabetes Reports·Alison H AffinatiAndrew T Kraftson
Jul 11, 2020·Chinese Medical Journal·Li-Yuan ZhouXin-Hua Xiao
Jul 2, 2019·Frontiers in Immunology·Avner LeshemEran Elinav
Oct 14, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ming SongCraig J McClain
May 31, 2019·Current Opinion in Nephrology and Hypertension·Menghan Liu, Lama Nazzal
Jul 25, 2019·Nature Microbiology·Manish KumarJens Nielsen
Nov 12, 2019·Frontiers in Microbiology·Amy D Willis
Jun 15, 2019·Current Obesity Reports·Jean DebédatJudith Aron-Wisnewsky
Feb 23, 2019·Expert Review of Gastroenterology & Hepatology·Tahli Singer-EnglarRuchi Mathur
Sep 6, 2018·Nature Reviews. Gastroenterology & Hepatology·Piriyah SinclairCarel W le Roux
Feb 12, 2021·Nature Communications·Frank A DucaTony K T Lam
Feb 9, 2021·Frontiers in Endocrinology·Ulrich DischingerFlorian Johannes David Seyfried
Mar 19, 2020·Cell Metabolism·Ruth E GimenoRandy J Seeley
Oct 30, 2019·Journal of Proteome Research·Naoaki ArimaMasanori Aikawa
Sep 16, 2021·Cell Reports·Fernando F AnhêJonathan D Schertzer

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Datasets Mentioned

BETA
PRJEB20172

Methods Mentioned

BETA
gastric bypass
PCR
454 sequencing

Software Mentioned

QIIME ( Quantitative Insights Into Microbial Ecology )
PyNAST
QIIME
UCLUST
GraphPad prism
UniFrac
GraphPad
Adonis
BLAST
ChimeraSlayer

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