Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1

Oxidative Medicine and Cellular Longevity
Han WuBiao Xu

Abstract

Oxidative stress is considered to be a critical factor in diabetes-induced endothelial progenitor cell (EPC) dysfunction, although the underlying mechanisms are not fully understood. In this study, we investigated the role of high mobility group box-1 (HMGB-1) in diabetes-induced oxidative stress. HMGB-1 was upregulated in both serum and bone marrow-derived monocytes from diabetic mice compared with control mice. In vitro, advanced glycation end productions (AGEs) induced, expression of HMGB-1 in EPCs and in cell culture supernatants in a dose-dependent manner. However, inhibition of oxidative stress with N-acetylcysteine (NAC) partially inhibited the induction of HMGB-1 induced by AGEs. Furthermore, p66shc expression in EPCs induced by AGEs was abrogated by incubation with glycyrrhizin (Gly), while increased superoxide dismutase (SOD) activity in cell culture supernatants was observed in the Gly treated group. Thus, HMGB-1 may play an important role in diabetes-induced oxidative stress in EPCs via a positive feedback loop involving the AGE/reactive oxygen species/HMGB-1 pathway.

References

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Citations

Feb 16, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Yeshuo MaAlex F Chen
Dec 15, 2019·International Journal of Molecular Sciences·Federico BiscettiAndrea Flex
Nov 17, 2016·Mediators of Inflammation·Han WuBiao Xu
Mar 17, 2021·Journal of Cellular and Molecular Medicine·Zheng ChenBiao Xu

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Methods Mentioned

BETA
ELISA
density gradient centrifugation
fluorescence microscopy
protein assay

Software Mentioned

BioRad
Quantity One
SPSS

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