Diabetes mellitus exacerbates post-myocardial infarction heart failure by reducing sarcolipin promoter methylation.

ESC Heart Failure
Zhongwei LiuJunkui Wang

Abstract

Sarcolipin (SLN) is a key regulator of sarcoplasmic reticulum calcium-ATPase (SERCA)2a, which handles intracellular calcium re-uptake. This study was aimed to investigate the involvement of SLN in post-myocardial infarction (MI) heart failure (HF) in diabetes. Diabetes/MI rat models were established. Altered SLN expression in diabetic hearts was screened out by microarray. A myocardiotropic viral vector was used to deliver siRNA to silence SLN. DNA methylation was evaluated by bisulfite sequencing. Cardiac functions were evaluated by invasive haemodynamic examinations. The SERCA2a activity, cytoplasmic calcium concentration ([Ca2+ ]i ), calcium spark, and myocyte contraction were detected. Correlation between HF and diabetes was analysed in a cohort consisted of 101 ST-segment elevated myocardial infarction (STEMI) patients between 2017 and 2019 [53.54 ± 4.64 years old; 61.4% male gender; HbA1c% 6.15 ± 2.00; and left ventricular ejection fraction (LVEF%) 40.64 ± 3.20%]. SLN expression was evaluated in left ventricular tissue sample from six STEMI patients complicated with diabetes and six STEMI patients without diabetes. Expressions of DNA methyltransferase 1a and DNA methyltransferase 3 were reduced in diabetic hearts, leading...Continue Reading

References

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Citations

Sep 11, 2020·ESC Heart Failure·Peng Zhong, Jianye Peng
Jul 29, 2021·Advanced Science·Sevgican DemirBilgen Ekim Üstünel
Aug 28, 2021·International Journal of Molecular Sciences·Nishadh RathodHoward S Young

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Methods Mentioned

BETA
transgenic
Fluorescence
Feature Extraction
transfections
PCR
electrophoresis
Protein Extraction
transfection
flow cytometry

Software Mentioned

SparkMaster Plugin
ImageJ
GeneSpring GX
PASW Statistics
IonWizard
Agilent Feature Extraction

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