Diabetic conditions modulate the adenosine monophosphate-activated protein kinase of podocytes

Kidney Research and Clinical Practice
Tae-Sun HaGi Dong Han

Abstract

Adenosine monophosphate-activated protein kinases (AMPKs), as a sensor of cellular energy status, have been known to play an important role in the pathophysiology of diabetes and its complications. Because AMPKs are known to be expressed in podocytes, it is possible that podocyte AMPKs could be an important contributing factor in the development of diabetic proteinuria. We investigated the roles of AMPKs in the pathological changes in podocytes induced by high-glucose (HG) and advanced glycosylation end products (AGEs) in diabetic proteinuria. We prepared streptozotocin-induced diabetic renal tissues and cultured rat and mouse podocytes under diabetic conditions with AMPK-modulating agents. The changes in AMPKα were analyzed with confocal imaging and Western blotting under the following conditions: (1) normal glucose (5mM, =control); (2) HG (30mM); (3) AGE-added; or (4) HG plus AGE-added. The density of glomerularphospho-AMPKα in experimental diabetic nephropathy decreased as a function of the diabetic duration. Diabetic conditions including HG and AGE changed the localization of phospho-AMPKα from peripheral cytoplasm to internal cytoplasm and peri- and intranuclear areas in podocytes. HG reduced the AMPKα (Thr172) phosphoryla...Continue Reading

Citations

Apr 25, 2018·Frontiers in Endocrinology·Anita A Wasik, Sanna Lehtonen
Apr 7, 2016·Journal of Korean Medical Science·Tae-Sun HaHee-Yul Ahn
Jul 19, 2021·Biochemical Pharmacology·Dorota Rogacka, Agnieszka Piwkowska

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Methods Mentioned

BETA
glycosylation
electrophoresis

Software Mentioned

IMAGEJ

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