Diabetic Csf1op/op mice lacking macrophages are protected against the development of delayed gastric emptying

Cellular and Molecular Gastroenterology and Hepatology
G CiprianiG Farrugia

Abstract

Diabetic gastroparesis is associated with changes in interstitial cells of Cajal (ICC), neurons and smooth muscle cells in both animal models and humans. Macrophages appear to be critical to the development of cellular damage that leads to delayed gastric emptying but the mechanisms involved are not well understood. Csf1op/op (Op/Op) mice lack biologically active Csf1, resulting in the absence of Csf1-dependent tissue macrophages. The aim of this study was to use Csf1op/op mice to determine the role of macrophages in the development of delayed gastric emptying. Animals were injected with streptozotocin to make them diabetic. Gastric emptying was determined weekly. Immunohistochemistry was used to identify macrophages and ICC networks in the gastric muscular layers. Oxidative stress was measured by serum malondialdehyde (MDA) levels. Quantitative, reverse transcription PCR was used to measure levels of mRNA. Csf1op/op mice had normal ICC. With onset of diabetes both Csf1op/op and wild type Csf1+/+ mice developed increased levels of oxidative stress (75.8 ± 9.1 and 41.2±13.6 nmol/mL MDA respectively). Wild type Csf1+/+ mice developed delayed gastric emptying after onset of diabetes (4/13) whereas no diabetic Csf1op/op mouse devel...Continue Reading

Citations

May 14, 2019·Endocrine Reviews·Adil E BharuchaDavid O Prichard
Mar 4, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Maham PervezJan D Huizinga
Sep 30, 2019·Gut·Madhusudan GroverVincenzo Stanghellini
Sep 5, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Madhusudan GroverGianrico Farrugia
Mar 21, 2020·Current Gastroenterology Reports·Amol SharmaHenry P Parkman
Aug 9, 2018·BMC Medical Genomics·Madhusudan GroverUNKNOWN NIDDK Gastroparesis Clinical Research Consortium (GpCRC)
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Oct 27, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S T EisenmanG Farrugia
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Jan 2, 2021·Gut·Maria Francesca Viola, Guy Boeckxstaens
Jan 24, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Shuangning SongShi Liu
Apr 6, 2016·Cellular and Molecular Gastroenterology and Hepatology·Gianluca CiprianiGianrico Farrugia
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Methods Mentioned

BETA
dissection
MDA

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