PMID: 9169602May 1, 1997Paper

Diacylglycerol generated by exogenous phospholipase C activates the mitogen-activated protein kinase pathway independent of Ras- and phorbol ester-sensitive protein kinase C: dependence on protein kinase C-zeta

The Biochemical Journal
M C van DijkW J Van Blitterswijk

Abstract

The role of diacylglycerol (DG) formation from phosphatidylcholine in mitogenic signal transduction is poorly understood. We have generated this lipid at the plasma membrane by treating Rat-1 fibroblasts with bacterial phosphatidylcholine-specific phospholipase C (PC-PLC). This treatment leads to activation of mitogen-activated protein kinase (MAPK). However, unlike platelet-derived growth factor (PDGF) or epidermal growth factor (EGF), PC-PLC fails to activate Ras and to induce DNA synthesis, and activates MAPK only transiently (<45 min). Down-regulation of protein kinase C (PKC) -alpha, -delta and -epsilon isotypes has little or no effect on MAPK activation by either PC-PLC or growth factors. However, Ro 31-8220, a highly selective inhibitor of all PKC isotypes, including atypical PKC-zeta but not Raf-1, blocks MAPK activation by PDGF and PC-PLC, but not that by EGF, suggesting that atypical PKC mediates the PDGF and PC-PLC signal. In line with this, PKC-zeta is activated by PC-PLC and PDGF, but not by EGF, as shown by a kinase assay in vitro, using biotinylated epsilon-peptide as a substrate. Furthermore, dominant-negative PKC-zeta inhibits, while (wild-type) PKC-zeta overexpression enhances MAPK activation by PDGF and PC-PL...Continue Reading

Citations

Mar 1, 2000·British Journal of Pharmacology·G LenzJ T Neary
Aug 28, 2003·Journal of Cellular Physiology·Antonella MuscellaSanto Marsigliante
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiD Schubert
Oct 29, 2000·The Journal of Biological Chemistry·M M KavurmaL M Khachigian
Jan 18, 2003·International Journal of Cancer. Journal International Du Cancer·Christopher Y ThomasRobert Wharen
May 3, 2003·Journal of Experimental Therapeutics & Oncology·Nicolas MaestreJean-Pierre Jaffrézou
Nov 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Jean-Hugues ParmentierKafait U Malik
Nov 16, 2007·The Biochemical Journal·Elisabetta AlbiMariapia Viola Magni
Apr 10, 1999·The Journal of Biological Chemistry·S FrutosM T Diaz-Meco
May 31, 2007·The Journal of Cell Biology·Leonie van ZeijlWouter H Moolenaar
Dec 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Honglei LiuHenry Jay Forman
Jan 1, 2013·Molecular Neurobiology·Haviryaji S G KalluriRobert J Dempsey
Mar 20, 1999·The Journal of Biological Chemistry·R P DoornbosP M van Bergen en Henegouwen
Nov 28, 2001·The Journal of Biological Chemistry·Nicole A BourbonMark Kester
Aug 26, 1998·FEMS Immunology and Medical Microbiology·S GhoshS Majumdar
May 5, 2001·Pharmacology & Therapeutics·C R Weinstein-OppenheimerJ A McCubrey
Jun 1, 2001·Advances in Enzyme Regulation·J MoscatM T Diaz-Meco
Jun 8, 1999·Chemistry and Physics of Lipids·W J van Blitterswijk, B Houssa
Nov 19, 2003·Biochemical and Biophysical Research Communications·Michael LeeWayne B Anderson
Apr 24, 2008·Journal of Plant Physiology·Erika KovácsAron Keresztes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.