PMID: 7892111Dec 1, 1994Paper

Diacylglycerol-induced activation of protein kinase C attenuates Na+ currents by enhancing inactivation from the closed state

Pflügers Archiv : European journal of physiology
C M Godoy, S Cukierman

Abstract

The causes of attenuation of Na+ currents by diacylglycerol (DAG)-induced protein kinase C (PKC) activation in mouse neuroblastoma N1E-115 cells were investigated using the cell-attached patch, and the perforated-patch (nystatin based) whole-cell voltage-clamp techniques. Activation of PKC by DAG attenuated Na+ currents. Attenuation occurred in the absence of significant changes in the time-course of Na+ currents. However, the steady-state inactivation curve of these currents shifted to more negative voltages by approximately 20 mV. Here we demonstrate that the time-course of inactivation is accelerated by treatment with DAG-like substances in a voltage-dependent manner (time constant of inactivation decreased by 2- and 3.6-fold at -60, and -30 mV, respectively). In cell-attached patches, treatment with DAG compounds increased the percentage of current traces showing no single Na+ channel openings in response to depolarizing voltage-clamp pulses. Moreover, the average of current traces containing single Na+ channel openings was essentially the same in control conditions and after treatment with DAG compounds. Removal of Na+ channel inactivation by the alkaloid batrachotoxin prevented the attenuation of Na+ currents by PKC activ...Continue Reading

References

Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
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Oct 1, 1992·Physiological Reviews·W A Catterall
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Jun 1, 1994·The Journal of Membrane Biology·C M Godoy, S Cukierman
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y QuW A Catterall

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Citations

Dec 4, 2013·Journal of Biosciences·Youn Kyoung SonWon Sun Park
Apr 1, 1997·Progress in Neuro-psychopharmacology & Biological Psychiatry·G Riedel
Sep 12, 2008·The European Journal of Neuroscience·Ulrike WasnerUlf Strauss
Dec 6, 2003·Journal of Neurophysiology·Kausalia VijayaragavanMohamed Chahine
Jun 5, 2001·Nature Reviews. Neuroscience·A R Cantrell, W A Catterall

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