Diaglycerol biosynthesis in everted sacs of rat intestinal mucosa.

Canadian Journal of Biochemistry
W C Breckenridge, A Kuksis

Abstract

The molecular specificity in the biosynthesis of diacylglycerols by rat intestinal mucosa was examined by means of radioactive markers, thin-layer chromatography with silver nitrate and gas-liquid chromatography with radioactivity monitoring. Bile salt micelles of alternately labeled monoacyglycerols and free fatty acids were incubated with everted sacs of intestinal mucosa for various periods of time and the diacylglycerols were isolated by solvent extraction and thin-layer chromatography. Sterospecific analyses of the X-1,2-diacylglycerols labeled from 2-monoacylglycerols showed that the sn-1,2-isomers (45-55%) were slightly in excess of the sn-2,3-isomers (34-45%) with the X-1,3-diacylglycerols accounting for the rest of the radioactivity (5-10%). This suggests that racemic diacylglycerols may be intermediates in the resynthesis of dietary fat in rat intestinal mucosa. Detailed analyses of the molecular species of the sn-1,2-diacylglycerols labeled from free fatty acids revealed that 10-45% of the total did not contain the acid present in the 2-monoacylglycerol supplied, and therefore had originated from the phosphatidic acid pathway. These findings are at variance with those obtained in isolated microsomes, which have sugge...Continue Reading

Citations

Dec 1, 1979·Environmental Health Perspectives·A KuksisA G Hoffman
Feb 11, 2003·The Journal of Biological Chemistry·Jingsong CaoYuguang Shi
Jan 11, 2005·The American Journal of Clinical Nutrition·Marie-Pierre St-Onge
Jun 20, 1986·Journal of Chromatography·A Kuksis, J J Myher
Jan 1, 1996·Progress in Lipid Research·R Lehner, A Kuksis
Apr 18, 1980·Biochimica Et Biophysica Acta·C M Lok, D J van der Horst

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