PMID: 2509565Oct 24, 1989Paper

Diagnosing endocarditis with the cloned 112 kDa antigen of Enterococcus faecalis

Journal of Immunological Methods
J P Burnie, I Clark

Abstract

A new indirect ELISA is presented for the diagnosis of enterococcal endocarditis. It is based on the cloning of Enterococcus faecalis DNA into lambda gt11. The library was screened by antisera from three cases of E. faecalis endocarditis. Three positive clones were found, all of which cross-reacted with the 112 kDa antigen of E. faecalis. One of these was taken and lysogenised into E. coli Y1089 to produce a fusion protein of 125 kDa. This IPTG dependent protein was purified by affinity chromatography and used in an indirect ELISA. The test differentiated between five cases of enterococcal endocarditis (IgG ELISA optical density greater than 0.8) and patients with endocarditis due to staphylococci (IgG ELISA optical density less than 0.243) and other streptococci (IgG ELISA optical density less than 0.656). Patients with a simple E. faecalis septicaemia had a maximum IgG ELISA optical density of 0.636. The only major cross-reaction occurred in patients with Streptococcus bovis endocarditis.

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Citations

Oct 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A SulaimanB E Murray
Jul 10, 2003·The Journal of Infection·R W WatkinT S J Elliott
Sep 5, 2001·The Journal of Infection·M ConnaughtonT S Elliott
Dec 2, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A KjerulfN Høiby
Jun 1, 1992·Journal of Clinical Pathology·R C Matthews, J P Burnie
Mar 31, 2015·Herz·D Horstkotte, C Piper
Oct 2, 2019·Frontiers in Cellular and Infection Microbiology·Florent ArregleDidier Raoult
Mar 1, 1994·Infection and Immunity·R C ArduinoR M Rakita

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