Diagnosis and therapy with CRISPR advanced CRISPR based tools for point of care diagnostics and early therapies

Gene
Vanita UppadaGirish Kumar Rasineni

Abstract

Molecular diagnostics is of critical importance to public health worldwide. It facilitates not only detection and characterization of diseases, but also monitors drug responses, assists in the identification of genetic modifiers and disease susceptibility. Based upon DNA variation, a wide range of molecular-based tests are available to assess/diagnose diseases. The CRISPR-Cas9 system has recently emerged as a versatile tool for biological and medical research. In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation. As designing CRISPR-guided nucleases can be done easily and relatively fast, the CRISPR/Cas9 system has evolved as widely used DNA editing tool. This technique led to a large number of gene editing studies in variety of organisms. CRISPR/Cas9-mediated diagnosis and therapy has picked up pace due to specificity and accuracy of CRISPR. The aim is not only to identify specific pathogens, especially virus but also to repair disease-causing alleles by changing the DNA sequence at the exact location on the chromosome. At present, PCR-based molecular diagnostic testing predominates; however, alternative technologies aimed at reducing genome complexi...Continue Reading

Citations

Mar 25, 2019·Molecular Diagnosis & Therapy·Roberto Gambari, Marina Kleanthous
Sep 18, 2020·Biological Procedures Online·Somayeh Jolany VangahGholamreza Ahmadian
Aug 25, 2020·Multimedia Tools and Applications·Abdullahi Umar IbrahimMehmet Ozsoz
Oct 1, 2019·Current Gene Therapy·Ling-Yan ZhouTing Xu
Nov 6, 2020·Journal of Clinical Microbiology·Mujahed I Mustafa, Abdelrafie M Makhawi
Jun 25, 2018·Fertility and Sterility·Laura H Goetz, Nicholas J Schork
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Sep 22, 2021·PloS One·Estel Aparicio-PratRobert Stanton
Feb 20, 2020·Molecular Therapy. Nucleic Acids·Tristan ScottKevin V Morris

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