Diagnosis of acute canine leptospirosis using multiple laboratory tests and characterization of the isolated strains

BMC Veterinary Research
Bruno Alonso MiottoM K Hagiwara

Abstract

Dogs presenting with acute leptospirosis may present non-specific clinical and laboratory findings, and the definitive diagnosis may require additional confirmatory tests, including bacterial culture, for the direct or indirect identification of the pathogen. The present study describes the diagnosis of leptospirosis in suspected dogs based on the use of multiple diagnostic tests, including serological, molecular and bacteriological tests, along with the characterization of the recovered leptospiral strains. Urine, serum and blood samples were collected from 33 dogs with suspected clinical leptospirosis treated at the University of São Paulo Veterinary Hospital Service (Hovet FMVZ-USP) between 2013 and 2016. Only dogs with high blood urea nitrogen and creatinine levels in association with multiple clinical manifestations of the disease were included. Leptospiral culture, PCR and serology (Microscopic agglutination test - MAT) were performed in blood and urine samples taken from all suspected dogs at clinical presentation, and an additional prospective MAT titration was performed in seven dogs. Infection could be identified exclusively by PCR in 10 dogs (30.3%), exclusively by MAT in four dogs (12.1%) and by both tests in four d...Continue Reading

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Citations

Nov 5, 2019·Transboundary and Emerging Diseases·Ana S FerreiraJoão Inácio
Nov 5, 2019·Frontiers in Veterinary Science·Lilia GutierrezHector Sumano
Mar 12, 2021·Comparative Immunology, Microbiology and Infectious Diseases·C S DiasM H Pinna
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Feb 1, 2022·Vector Borne and Zoonotic Diseases·Tarindi AthapattuChandika Gamage

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Datasets Mentioned

BETA
AY996800
MG640117
MG640121

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BIOEDIT Sequence Alignment Editor
CEUAVET
SPSS Statistics
BLAST

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