Diagnosis of aerobic vaginitis by quantitative real-time PCR

Archives of Gynecology and Obstetrics
T A RumyantsevaGilbert G G Donders

Abstract

To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test. Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus agalactiae, S. aureus; Lactobacillus spp. AV diagnosis obtained by wet-mount microscopy was used as reference. Some level of AV was diagnosed in 23 (23.7 %) cases. Various concentrations of Enterobacteriacea, Staphylococcus spp., Streptococcus spp. were detected an all patients. Enterococcus spp. were detected in 76 (78.3 %) cases. Summarized concentrations of aerobes were tenfold higher in AV-positive compared to AV-negative cases [7.30lg vs 6.06lg (p = 0.02)]. Concentrations of aerobes in severe, moderate and light AV cases did not vary significantly (p = 0.14). Concentration of lactobacilli was 1000-fold lower in AV-positive cases compared to normal cases (5.3lg vs 8.3lg, p < 0.0001). Streptococcus spp. dominated in the majority of AV-positive cases [19/22 (86.4 %) samples]. The relation of high loads of aerobes to the low numbers of Lactobacilli are a reliable marker for the...Continue Reading

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Citations

Jun 9, 2016·Sexually Transmitted Infections·Mark J Mason, Andrew J Winter
Jul 22, 2017·Research in Microbiology·Tania Crucitti
Sep 25, 2017·Obstetrics and Gynecology·Gilbert Donders, Pedro Vieira Baptista
Dec 21, 2017·Infectious Diseases in Obstetrics and Gynecology·Pedro Vieira-BaptistaGilbert Donders
Apr 5, 2020·Bulletin of Experimental Biology and Medicine·O V BudilovskayaA M Savicheva
Jul 9, 2020·Frontiers in Cellular and Infection Microbiology·David Pacha-HerreraAntónio Machado
Sep 28, 2021·Frontiers in Cellular and Infection Microbiology·Kwan Young OhJun Sook Park
May 16, 2017·Research in Microbiology·Gilbert G G DondersPedro Vieira-Baptista

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