Diagnosis of arthritis using near-infrared fluorochrome Cy5.5

Investigative Radiology
Andreas HanschWerner A Kaiser

Abstract

Near-infrared range fluorescence (NIRF) imaging is a potential tool to diagnose biologic processes in vivo. This applicability study sought to define whether imaging with fluorochrome Cy5.5 can identify arthritis in murine antigen-induced arthritis (AIA). On day 7 of AIA (n = 9 mice), fluorescence intensities in inflamed and contralateral knee joints (the latter as internal control) were measured before and after intravenous injection of Cy5.5 (until 72 hours). Cy5.5 joint deposition was verified by confocal laser-scanning microscopy. Dye phagocytosis was evaluated in cultured macrophages (cell line PMJ2-R) by FACS analysis. Cy5.5 binding to serum protein was tested by NIRF scanning and gel electrophoresis. Between 2 and 72 hours, the arthritic knee joints showed significantly higher fluorescence intensities compared with contralateral joints. Microscopy confirmed Cy5.5 deposition in the synovial membrane. Cultured macrophages actively phagocytosed Cy5.5. Cy5.5 bound mainly to albumin as the main serum protein. NIRF imaging with Cy5.5 can identify arthritic joints in vivo, likely due to nonspecific deposition.

References

Mar 1, 1983·Collagen and Related Research·H G Fassbender
Jan 1, 1996·Arthritis and Rheumatism·D MulherinB Bresnihan
Sep 1, 1995·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·J R Levick
May 18, 1999·Rheumatology·R J WakefieldP Emery
Nov 30, 2000·Arthritis Research·R W KinneG R Burmester
Dec 7, 2000·Journal of Microscopy·K König
Apr 3, 2001·Nature Biotechnology·A BeckerC Grötzinger
Apr 27, 2001·Radiology·R Weissleder, U Mahmood
May 9, 2001·The Journal of Clinical Investigation·Y NikiN Tada
Oct 10, 2001·Journal of Autoimmunity·J SimonR Bräuer
Feb 13, 2002·Arthritis and Rheumatism·Henk VisserJohanna M W Hazes
Sep 10, 2003·Investigative Radiology·Andreas HanschWerner A Kaiser
Mar 17, 2004·Arthritis and Rheumatism·Andreas HanschWerner A Kaiser
May 1, 2004·European Radiology·Ingrid HilgerWerner A Kaiser

❮ Previous
Next ❯

Citations

Jun 14, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Gerhard H SimonBernd J Pichler
Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
Sep 26, 2012·Nature Reviews. Rheumatology·James M MountzJohn D Mountz
Mar 27, 2010·Rheumatology·Luke L GompelsEwa M Paleolog
Oct 19, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Thomas Krucker, Britto S Sandanaraj
Jan 11, 2007·Journal of Biomedical Optics·Marcus-René LisyIngrid Hilger
Mar 26, 2011·Thrombosis and Haemostasis·E Kenne, L Lindbom
Nov 29, 2008·Journal of Translational Medicine·Reinhard MeierHeike E Daldrup-Link
Feb 25, 2011·Arthritis Research & Therapy·Luke L Gompels, Ewa M Paleolog
Jan 17, 2014·Arthritis Research & Therapy·Valentin S SchäferBoris Ehrenstein
Jun 26, 2013·AJR. American Journal of Roentgenology·Andrew WilmotPhilipp Mayer-Kuckuk
Aug 10, 2011·International Journal of Clinical Rheumatology·Daniel GolovkoHeike Daldrup-Link
May 5, 2007·Journal of Biomedical Optics·Kristen E AdamsEva M Sevick-Muraca
Jan 5, 2010·Trends in Biotechnology·Marie K ReumannPhilipp Mayer-Kuckuk
Dec 9, 2009·Academic Radiology·Thomas FischerKay-Geert A Hermann
Nov 1, 2008·Contrast Media & Molecular Imaging·Sophie BoddingtonHeike Daldrup-Link
Apr 17, 2008·Lasers in Surgery and Medicine·Andreas HanschWerner A Kaiser
Aug 8, 2006·ChemMedChem·Xavier MontetRalph Weissleder
Jun 15, 2016·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Yongmin ChangYoung Mo Kang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.