Diagnosis of congenital toxoplasmosis by using a whole-blood gamma interferon release assay.

Journal of Clinical Microbiology
Emmanuelle ChapeyFrançois Peyron

Abstract

Congenital toxoplasmosis in newborns is generally subclinical, but infected infants are at risk of developing ocular lesions. Diagnosis at birth relies mainly on serological tests. Cell-mediated immunity plays the major role in resistance to infection but is not routinely investigated for diagnostic purposes. Here, we describe a simple test based on the gamma interferon (IFN-gamma) response after stimulation of whole blood by crude parasitic antigens. One milliliter of heparinized blood was centrifuged; plasma was kept for routine serological tests, and pellets were resuspended in culture medium. After 24 h of culture in the presence of crude Toxoplasma gondii antigen, the cells were centrifuged and the supernatant was assayed for IFN-gamma. For 62 infants under 1 year of age born to mothers who were infected during pregnancy, the sensitivity and specificity of the test were 94% (with positive results for 16 of 17 infected infants) and 98% (with negative results for 44 of 45 uninfected infants), respectively. The false-negative result was for a treated baby who gave positive results after the withdrawal of treatment. The false positive was obtained for a 3-month-old baby. For a cohort of 124 congenitally infected patients betwe...Continue Reading

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Citations

Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Feb 28, 2014·Immunobiology·Om Prakash Singh, Shyam Sundar
Jan 15, 2014·Progress in Retinal and Eye Research·M MaenzU Pleyer
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Aug 25, 2015·Experimental Parasitology·Qing YinXun Suo
Sep 23, 2014·International Journal of Medical Microbiology : IJMM·Dirk SchlüterCarsten Lüder
Nov 17, 2011·Acta Tropica·Yves CarlierFrançois Peyron
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Dec 19, 2016·Experimental Parasitology·Shima MahmoudiHossein Keshavarz
Nov 25, 2017·Parasite Immunology·L PetroneD Goletti
Sep 3, 2010·Current Opinion in Ophthalmology·Sivakumar R Rathinam, Kawali A Ashok
Aug 30, 2014·Journal of Biomolecular Screening·Alessandra Ricciardi, Momar Ndao
Feb 8, 2020·Parasite : Journal De La Société Française De Parasitologie·Loïc SimonUNKNOWN Toxoplasma p35 Study Group
Sep 8, 2011·International Ophthalmology Clinics·Miriam Englander, Lucy H Y Young
Jan 19, 2016·The Pediatric Infectious Disease Journal·Emmanuelle ChapeyFrançois Peyron
Oct 9, 2020·Scientific Reports·Thádia Evelyn de AraújoUNKNOWN UFMG Congenital Toxoplasmosis Brazilian Group UFMG-CTBG, beside the authors
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Aug 20, 2019·Deutsches Ärzteblatt International·Uwe PleyerFrank Seeber
Nov 24, 2019·Revista iberoamericana de micología·Marcela Rubio-CarrasquillaErnesto Moreno
Aug 10, 2021·International Ophthalmology·Dimitrios KalogeropoulosChris Kalogeropoulos
Apr 6, 2019·ACS Infectious Diseases·Matthew H Collins, Jesse J Waggoner

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