PMID: 8944606Nov 1, 1996Paper

Diagnosis of cutaneous tuberculosis in biopsy specimens by PCR and southern blotting

Journal of Clinical Pathology
E QuirósG Piédrola

Abstract

To evaluate the use of a gene amplification and hybridisation method for detecting mycobacterial nucleic acid as a possible diagnostic method for cutaneous tuberculosis infection. Biopsy specimens from 20 patients with various skin conditions of possible tuberculous aetiology were studied. Six patients had ulcerative nodules, seven lupiform lesions, two non-necrotic granulomas, one scrofulous lichen, one impetigo, one erythematosus lesions, one warty lesions, and one suspected tuberculous lipoma. Biopsy specimens were stained using Ziehl-Neelsen stain and cultured in Lowenstein-Jensen medium. DNA was extracted and then amplified by PCR using primers specific for the Mycobacterium tuberculosis complex. Specificity was confirmed by Southern blotting. Of the specimens, 30% were positive for mycobacteria on staining with Ziehl-Neelsen stain, 60% were culture positive and 85% PCR positive. Only 35.2% of specimens were positive with all three techniques. A further 32.5% were both culture and PCR positive. All PCR negative samples were also negative when cultured or stained with Ziehl-Neelsen stain. Of the PCR positive specimens, 29.4% were negative when cultured or stained. PCR, using suitable primers, is an efficient and sensitive m...Continue Reading

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Citations

Jul 25, 2000·Journal of Clinical Laboratory Analysis·E QuirósM C Maroto
May 8, 1999·Clinical and Experimental Dermatology·A LeeK Lee
Nov 22, 2012·The American Journal of Dermatopathology·Ossama Abbas, Jag Bhawan
Oct 26, 2005·Journal of the American Academy of Dermatology·Karan K SraStephen K Tyring
Aug 12, 2016·Indian Journal of Pathology & Microbiology·Ilhan Afsar, Fatma Sule Afsar
Jul 14, 2005·Scandinavian Journal of Infectious Diseases·A ZampettiP L Amerio
Oct 23, 1997·Nature·P Sassone-Corsi
Jun 13, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·J Y LiC S Ng
Aug 17, 2018·Dermatology Research and Practice·Priyatam KhadkaJanuka Thapaliya
Feb 25, 2017·Microbiology Spectrum·Michael K Hill, Charles V Sanders
Jan 1, 2009·Medical Journal, Armed Forces India·P KinraA Kumar

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