Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests

Avian Pathology : Journal of the W.V.P.A
E FringuelliD Todd

Abstract

Development of the first conventional and real-time polymerase chain reaction (PCR) tests for the diagnoses of duck circovirus (DuCV) infections is described. Both tests amplified a 230 bp fragment specific to the DuCV Rep gene. Although both tests had the same detection limit (13 x 10(3) target DNA/ml) when the target DNA was diluted in water, the detection limit of the real-time test (13 x 10(4) target DNA/ml) was 10-fold less than the conventional test (13 x 10(5) target DNA/ml) when the amplifications were performed in the presence of cellular DNA. Using the conventional PCR test, DuCV DNA was detected in 85 (84%) of 101 bursa of Fabricius samples from dead or sick ducks, aged between 1 and 12 weeks, and in samples from 35 (94%) of 37 flocks. Application of the SYBR Green-based real-time PCR test to 54 selected bursa of Fabricius samples indicated that more samples were positive by real-time PCR than by conventional PCR, allowed the numbers of genome copies to be estimated and showed that some bursa of Fabricius samples contained over 10(13) genome copies/g tissue. Although DuCV infections were detected in birds aged from 1 to 12 weeks, higher virus DNA levels were detected in ducks aged older than 5 weeks than in ducks you...Continue Reading

References

Jan 16, 1999·Journal of Clinical Microbiology·M MartellJ Guardia
Nov 29, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J A SmythD Todd
Dec 4, 2003·Archives of Virology·K HattermannA Mankertz
Jul 6, 2004·The Veterinary Record·D SoikeA Mankertz
Oct 1, 2000·Avian Pathology : Journal of the W.V.P.A·D Todd

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Citations

Jun 12, 2012·Virologica Sinica·Xing-xiao ZhangShi-jin Jiang
Aug 1, 2012·Avian Pathology : Journal of the W.V.P.A·M BiđinD Majnarić
Jun 15, 2012·Virology Journal·Grzegorz WoźniakowskiElżbieta Samorek-Salamonowicz
Jul 29, 2011·Avian Diseases·Guanghua FuJiansheng Lin
Dec 25, 2013·The Journal of Veterinary Medical Science·Se-Yeoun ChaHyung-Kwan Jang
Apr 28, 2012·Veterinary Microbiology·Qi-Wang XiangShi-Jin Jiang
Aug 17, 2010·Veterinary Microbiology·Dan WangDabing Zhang
Aug 7, 2013·Science China. Life Sciences·GuangCheng XieZhaoJun Duan
Apr 23, 2010·Veterinary Microbiology·Shao-Ning LiuShi-Jin Jiang
May 31, 2015·Virology Journal·Pengfei LiAnchun Cheng
Sep 30, 2014·Veterinary Microbiology·Zhiguo LiShijin Jiang
Nov 1, 2016·Genome Announcements·Wenchao SunNingyi Jin
Apr 28, 2007·Avian Diseases·Alejandro BandaKarel A Schat
Jun 13, 2020·Transboundary and Emerging Diseases·Jun JiQingmei Xie

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Datasets Mentioned

BETA
AY228555
AJ964962

Methods Mentioned

BETA
PCR
electrophoresis
electron microscopy
dot blot

Software Mentioned

EXCEL
iCycler
Clustal W

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