Diagnosis of Echinococcus multilocularis infection by reverse-transcription polymerase chain reaction

Gastroenterology
Peter KernM Frosch

Abstract

Alveolar echinococcosis is a life-threatening parasitosis occurring in countries in the Northern hemisphere. The diagnosis of an Echinococcus multilocularis infection is routinely performed by radiological techniques and by the detection of specific antibodies in the sera of infected patients. However, because serodiagnosis fails in 5%-10% and radiological techniques are difficult to interpret in some cases, we developed a polymerase chain reaction for the detection of echinococcal-specific messenger RNA from fine-needle biopsy specimens. This technique was applied to the diagnosis of alveolar echinococcosis in a 20-year-old seronegative woman. Detection of messenger RNA not only allowed the diagnosis of echinococcal disease but also proved to be a reliable measure for determining the efficacy of an antiparasitic therapy.

References

Aug 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J F WilsonP M Schantz
Oct 1, 1991·Molecular and Biochemical Parasitology·P M FroschD Bitter-Suermann
Mar 1, 1995·Infection·H D NothdurftT Löscher

❮ Previous
Next ❯

Citations

Jan 6, 1999·Journal of Hepatology·R W AmmannJ Eckert
Jun 26, 2001·Tropical Medicine & International Health : TM & IH·M M Siles-Lucas, B B Gottstein
May 30, 2013·Antimicrobial Agents and Chemotherapy·Tatiana KüsterAndrew Hemphill
Jan 17, 2004·Clinical Microbiology Reviews·Johannes Eckert, Peter Deplazes
Dec 8, 2004·Journal of Clinical Microbiology·Sophie GeorgesErmanno Candolfi
Jul 18, 2006·Der Internist·I SchiefkeS Schubert
Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S ReuterP Kern
May 15, 1998·Mayo Clinic Proceedings·H A Carpenter
Sep 24, 2015·Acta Tropica·Norbert Georg SchwarzHagen Frickmann
Jan 3, 2006·Parasitology International·Peter M Schantz
Apr 14, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D CarmenaE Eraso
May 31, 2016·Acta Tropica·Akira ItoEric Hoberg
May 21, 1998·Antimicrobial Agents and Chemotherapy·H JuraM Frosch
Jun 20, 1998·Clinical Microbiology Reviews·C N Powers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.