Diagnosis of Helicobacter pylori by carbon-13 urea breath test using a portable mass spectrometer

SAGE Open Medicine
J SreekumarAjm Watson

Abstract

In the non-invasive detection of markers of disease, mass spectrometry is able to detect small quantities of volatile markers in exhaled air. However, the problem of size, expense and immobility of conventional mass spectrometry equipment has restricted its use. Now, a smaller, less expensive, portable quadrupole mass spectrometer system has been developed. Helicobacter pylori has been implicated in the development of chronic gastritis, gastric and duodenal ulcers and gastric cancer. To compare the results obtained from the presence of H. pylori by a carbon-13 urea test using a portable quadrupole mass spectrometer system with those from a fixed mass spectrometer in a hospital-based clinical trial. Following ethical approval, 45 patients attending a gastroenterology clinic at the Royal Liverpool University Hospital exhaled a breath sample into a Tedlar gas sampling bag. They then drank an orange juice containing urea radiolabelled with carbon and 30 min later gave a second breath sample. The carbon-13 content of both samples was measured using both quadrupole mass spectrometer systems. If the post-drink level exceeded the pre-drink level by 3% or more, a positive diagnosis for the presence of H. pylori was made. The findings we...Continue Reading

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Citations

Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours
Jun 3, 2021·International Journal of Molecular Sciences·María Fernanda GonzálezAndrew F G Quest

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

MATLAB
QMS

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