Diagnosis of Helicobacter pylori recurrence: relapse or reinfection? Usefulness of molecular tools

Scandinavian Journal of Gastroenterology
Josette RaymondCatherine Dauga

Abstract

Infection due to Helicobacter pylori causes many gastrointestinal diseases including peptic ulcers and gastric carcinoma. Their treatment and prevention depends on the successful eradication of H. pylori. However, even after a well-conducted treatment, H. pylori persists in about 10-30% of patients. Recurrent infections can correspond to relapse or to re-infection and require appropriate medical care. In this study, we explore retrospectively three clinical cases using molecular methods, and propose new guidelines for the diagnosis of recurrence. Ten colonies of H. pylori were selected from the primary culture of biopsy samples taken from the antrum and fundus for each patient. The genotype of each isolated colony was determined by analyzing the polymorphism of two housekeeping genes, hspA and glmM. The genome-wide composition of H. pylori strains was studied using in house macro-arrays designed. Relapses were demonstrated by the stability of genotypes and the slight genetic variability of strains on macro-arrays. Two patients suffered from relapses, one and three years after H. pylori treatment. For the third patient, both the polymorphism of glmM and hspA genotypes and the diversity of CDSs identified on macro-arrays suggeste...Continue Reading

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Citations

Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours
Mar 13, 2020·Emerging Microbes & Infections·Yong XieUNKNOWN Chinese Society of Gastroenterology, Chinese Study Group on Helicobacter pylori and Peptic Ulcer
Sep 20, 2019·Journal of Cellular and Molecular Medicine·Yan Sun, Jun Zhang
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Aug 2, 2017·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·D SmithJ A Carr
Aug 28, 2021·Journal of Clinical Medicine·Irena Mladenova

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