Diagnosis of human and canine Brucella canis infection: development and evaluation of indirect enzyme-linked immunosorbent assays using recombinant Brucella proteins

Heliyon
Miryan Margot Sánchez-JiménezMartha Olivera-Angel

Abstract

Brucella canis, a Gram-negative coccobacilli belonging to the genus Brucellae, is a pathogenic bacterium that can produce infections in dogs and humans. Multiple studies have been carried out to develop diagnostic techniques to detect all zoonotic Brucellae. Diagnosis of Brucella canis infection is challenging due to the lack of highly specific and sensitive diagnostic assays. This work was divided in two phases: in the first one, were identified antigenic proteins in B. canis that could potentially be used for serological diagnosis of brucellosis. Human sera positive for canine brucellosis infection was used to recognize immunoreactive proteins that were then identified by performing 2D-GEL and immunoblot assays. These spots were analyzed using MALDI TOF MS and predicted proteins were identified. Of the 35 protein spots analyzed, 14 proteins were identified and subsequently characterized using bioinformatics, two of this were selected for the next phase. In the second phase, we developed and validated an indirect enzyme-linked immunosorbent assays using those recombinant proteins: inosine 5' phosphate dehydrogenase, pyruvate dehydrogenase E1 subunit beta (PdhB) and elongation factor Tu (Tuf). These genes were PCR-amplified fro...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 24, 2002·Journal of Bacteriology·Michel EschenbrennerVito G DelVecchio
Nov 5, 2002·Veterinary Microbiology·Vito G DelVecchioCesar V Mujer
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Patricia Seco-MediavillaNieves Vizcaíno
Aug 23, 2003·Infection and Immunity·Araceli Contreras-RodriguezAhide Lopez-Merino
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Juliana CassataroPablo C Baldi
Apr 21, 2004·Protein Science : a Publication of the Protein Society·Chin-Sheng YuJenn-Kang Hwang
Jul 24, 2004·Animal Reproduction Science·M M Wanke
Aug 7, 2004·Journal of Clinical Microbiology·François J PicardMichel G Bergeron
Sep 11, 2004·Clinical and Diagnostic Laboratory Immunology·M Victoria DelpinoPablo C Baldi
Apr 13, 2005·Journal of Medical Microbiology·Nidia E LuceroNestor Jacob
Apr 13, 2005·Journal of Medical Microbiology·Nidia E LuceroIsabelle Jacques
Jun 3, 2005·The New England Journal of Medicine·Georgios PappasEpameinondas Tsianos
Jun 28, 2005·Nucleic Acids Research·J ChengP Baldi
Jan 24, 2006·Journal of Chemical Information and Modeling·Akbar S KhanVito G Delvecchio
Apr 26, 2006·Immunome Research·Jens Erik Pontoppidan LarsenMorten Nielsen
May 24, 2006·Theriogenology·R Bruce Hollett
Oct 27, 2006·FEMS Immunology and Medical Microbiology·Araceli Contreras-RodriguezAhide Lopez-Merino
Apr 20, 2007·Research in Veterinary Science·Stella Maria Barrouin-MeloSongelí Menezes Freire
Dec 6, 2008·BMC Bioinformatics·Julia PonomarenkoBjoern Peters
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Aug 6, 2009·Epidemiology and Infection·N E LuceroS M Ayala
Aug 10, 2010·Research in Veterinary Science·Maria Zoraida Daltro de OliveiraStella Maria Barrouin-Melo
Aug 11, 2010·International Journal of Antimicrobial Agents·Georgios Pappas
Aug 4, 2011·Expert Review of Anti-infective Therapy·Sascha Al Dahouk, Karsten Nöckler
Sep 17, 2011·Science China. Life Sciences·Zhongpeng ZhaoXiliang Wang
Sep 24, 2011·Annual Review of Microbiology·Vidya L AtluriRenée M Tsolis
Nov 2, 2011·The Veterinary Clinics of North America. Small Animal Practice·Chelsea L Makloski
Dec 24, 2011·Veterinary Microbiology·Kyung Yuk KoJi-Yeon Kim
Mar 1, 2012·Journal of Proteome Research·Vassilios SandalakisYiannis Tselentis
Mar 1, 2012·Bioinformatics·Konstantin OkonechnikovUNKNOWN UGENE team
Mar 1, 2012·FEMS Microbiology Reviews·Kristine von BargenSuzana P Salcedo

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Citations

Mar 20, 2021·Frontiers in Veterinary Science·Renato L SantosTatiane A Paíxão

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Datasets Mentioned

BETA
HG803175.1
HG803176.1

Methods Mentioned

BETA
ELISA
Polymerase Chain Reaction
Enzyme-Linked Immunosorbent
protein assay
electrophoresis
PCR
Assay
proteins assay
iELISA

Software Mentioned

SPSS
ImageScannerTM 2D Platinum
SCRATCH Protein Preditor
RSAT
BLASTP
excel
BepiPred
Ellipro
PSORTb
Mascot

Related Concepts

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Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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