Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cards.

The Journal of Molecular Diagnostics : JMD
Lori ScangaMargaret L Gulley

Abstract

Congenital human cytomegalovirus (HCMV) infection affects 1% of children and is the most common infectious cause of sensorineural hearing loss. Due to the difficulty of diagnosing deafness and other neurological disorders in infants, affected individuals may not be recognized until much later when active infection has resolved and culture is no longer informative. To overcome this problem, congenital HCMV infection was diagnosed retrospectively by testing residual blood samples collected from newborns and dried on perinatal cards as part of the North Carolina Newborn Screening Program. We modified the Qiagen method for purifying DNA from dried blood spots to increase the sample size and recovery of the lysate. A multiplex, real-time TaqMan polymerase chain reaction assay on an ABI 7900 instrument measured a highly conserved segment of the HCMV polymerase gene and the APOB human control gene. HCMV DNA was detected in blood dried on perinatal cards from all seven infants with culture-proven congenital infection, and all 24 negative control cases lacked detectable HCMV DNA. Our findings suggest that it is possible to diagnose congenital HCMV infection using dried blood collected up to 20 months earlier. Further studies are warrant...Continue Reading

References

Feb 1, 1994·Journal of Virological Methods·M ShibataK Hirai
Jun 1, 1993·The Journal of Infectious Diseases·K B BalcarekR F Pass
May 18, 1999·Archives of Disease in Childhood. Fetal and Neonatal Edition·B FischlerI Lewensohn-Fuchs
Jun 29, 2002·Journal of Clinical Microbiology·Jason L Sanchez, Gregory A Storch
May 12, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sandro BindaMaria Barbi

❮ Previous
Next ❯

Citations

Apr 15, 2010·JAMA : the Journal of the American Medical Association·Suresh B BoppanaUNKNOWN National Institute on Deafness and Other Communication Disorders CMV and Hearing Multicenter Screening (CHIMES) Study
Jun 10, 2010·Journal of Inherited Metabolic Disease·Sheila C DollardScott D Grosse
Jun 3, 2011·The New England Journal of Medicine·Suresh B BoppanaUNKNOWN National Institute on Deafness and Other Communication Disorders CHIMES Study
Jan 9, 2013·Clinical Microbiology Reviews·Sheetal ManicklalRavindra K Gupta
May 17, 2013·Journal of Clinical Microbiology·Ajit P LimayeKeith R Jerome
Sep 28, 2007·Journal of Clinical Microbiology·Christelle Vauloup-FellousMarianne Leruez-Ville
Apr 5, 2013·PloS One·Hans-Henrik M DahlJessica Sjahalam-King
Feb 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marianne Leruez-VilleJean-François Magny
Jun 29, 2012·Journal of Perinatology : Official Journal of the California Perinatal Association·F PiersigilliC Auriti
Jan 17, 2012·Antiviral Research·Ingrid J M SnijdewindRob A Gruters
Oct 6, 2009·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·M Leruez-VilleJ-F Magny
Sep 29, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jutte J C de VriesAnn C T M Vossen
Mar 14, 2008·Gynécologie, obstétrique & fertilité·G BenoistY Ville
Aug 13, 2011·Journal of Medical Virology·Jutte J C de VriesAnn C T M Vossen
Jan 20, 2011·Reviews in Medical Virology·Jutte J C de VriesBernard A M van der Zeijst
Jan 9, 2013·Journal of Medical Virology·Joppe NijmanMalgorzata A Verboon-Maciolek
Mar 17, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Joppe NijmanMalgorzata A Verboon-Maciolek
May 7, 2015·Gastroenterology Research and Practice·Łukasz SzylbergAndrzej Marszałek
Oct 13, 2009·The Pediatric Infectious Disease Journal·K Yeon ChoiMark R Schleiss
Apr 3, 2008·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Weihua TangMargaret L Gulley
Sep 16, 2015·Current Opinion in HIV and AIDS·Kobus HerbstTill Bärnighausen
May 18, 2012·The Pediatric Infectious Disease Journal·Joppe NijmanMalgorzata A Verboon-Maciolek
Feb 4, 2014·Expert Review of Molecular Diagnostics·Giulia PiccirilliTiziana Lazzarotto
Jan 5, 2008·The Pediatric Infectious Disease Journal·Naoki Inoue, Shin Koyano
Dec 11, 2019·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mina SmiljkovicFatima Kakkar
Jun 7, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Sylvain LehmannChristophe Hirtz
Oct 29, 2014·Pediatrics·Julie GoderisIngeborg Dhooge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Related Papers

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Maria BarbiD Melotti
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Sandro BindaMaria Barbi
© 2022 Meta ULC. All rights reserved