Diagnosis of local hepatic tuberculosis through next-generation sequencing: Smarter, faster and better

Clinics and Research in Hepatology and Gastroenterology
Jing-Wen AiWen-Hong Zhang

Abstract

A 45-year-old man who complained of continuous fever and multiple hepatic masses was admitted to our hospital. Repeated MRI manifestations were similar while each radiological report suggested contradictory diagnosis pointing to infections or malignances respectively. Pathologic examination of the liver tissue showed no direct evidence of either infections or tumor. We performed next-generation sequencing on the liver tissue and peripheral blood to further investigate the possible etiology. High throughput sequencing was performed on the liver lesion tissues using BGISEQ-100 platform, and data was mapped to the Microbial Genome Databases after filtering low quality data and human reads. We identified a total of 299 sequencing reads of Mycobacterium tuberculosis (M. tuberculosis) complex sequences from the liver tissue, including 8, 229 of 4,424,435 of the M. tuberculosis nucleotide sequences, and Mycobacterium africanum, Mycobacterium bovis, and Mycobacterium canettii were also detected due to the 99.9% identical rate among these strains. No specific Mycobacterial tuberculosis nucleotide sequence was detected in the sample of peripheral blood. Patient's symptom quickly recovered after anti-tuberculosis treatment and repeated Zi...Continue Reading

Citations

Nov 7, 2019·Critical Reviews in Microbiology·Dongsheng HanJinming Li
Jan 14, 2021·BMC Infectious Diseases·Hongxia DuanShuanshuan Xie
Dec 29, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Xi LiuLi Ding
Sep 27, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Wei WangQing Xie
Feb 26, 2021·Journal of the Neurological Sciences·Aiqing LinXueping Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.