Diagnosis of micrometastases by the amplification of tissue-specific genes

Gene
M NeumaierC Wagener

Abstract

Micrometastases of solid tumors are most commonly detected by immunocytochemistry using monoclonal antibodies directed against tissue-specific gene products like cytokeratin-18 (CK-18) and the carcinoembryonic antigen (CEA). While CK-18 is a marker for epithelia in general, CEA is mainly employed in the detection of gastrointestinal and breast carcinomas. To improve the sensitivity and specificity of micrometastasis detection, we planned to establish polymerase chain reaction (PCR) assays for both markers. Here we provide strong evidence for the existence of a CK-18 pseudogene, since specific amplification (i) was readily obtained from healthy bone marrow donors, (ii) did not require reverse transcription of CK-18 mRNA and (iii) was not abolished by RNase treatment. Using a CK-18-specific probe, Southern blot analyses revealed identical-size fragments for both genomic DNA and a CK-18 cDNA after digestion with appropriate restriction enzymes. On the other hand, the amplification of CEA mRNA (i) was never observed in bone marrow samples of healthy donors or patients without solid tumors, (ii) required intact mRNA and the reverse transcriptase reaction, and (iii) could not be obtained after RNase treatment. In reconstitution exper...Continue Reading

References

Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G SchlimokG Riethmüller
Jul 5, 1991·Science·M HollsteinC C Harris
Apr 30, 1991·Biochemical and Biophysical Research Communications·M KurokiY Matsuoka
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G SchlimokG Riethmüller
Apr 27, 1989·Nature·W R Brown
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M VasseurF Jacob
Jan 1, 1988·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·J Thompson, W Zimmermann
May 1, 1988·Mutation Research·J L Bos
Jan 1, 1985·Critical Reviews in Oncology/hematology·J E Shively, J D Beatty
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

❮ Previous
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Citations

Dec 11, 1996·International Journal of Cancer. Journal International Du Cancer·M MoriT Akiyoshi
Nov 24, 1999·Diseases of the Colon and Rectum·D García-OlmoJ Cifuentes
Jan 8, 2003·Pathology Oncology Research : POR·József TímárLászló Kopper
Mar 11, 2006·International Journal of Colorectal Disease·Khaled M MadboulyVictor W Fazio
Jun 28, 1995·Journal of Immunological Methods·C GriwatzK S Zänker
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Angelo AquinoEnzo Bonmassar
Aug 31, 1999·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·M FleischhackerK Possinger
Oct 26, 1999·Cancer Letters·M StimpflR Zeillinger
Apr 14, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·N BeroisE Osinaga
Oct 2, 2001·The British Journal of Surgery·G TsavellasT G Allen-Mersh
Dec 21, 2000·The British Journal of Surgery·M R KellH P Redmond
Jul 23, 1998·The New England Journal of Medicine·G J LiefersR A Tollenaar
Mar 21, 1998·Journal of Hematotherapy·A A Ross
Jul 7, 1999·Journal of the National Cancer Institute·K PantelO Fodstad
Jun 4, 2005·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·O J O'ConnorH P Redmond
Jul 19, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Ilka VogelBernd Kremer
Apr 9, 2004·Annals of Surgical Oncology·Timothy M PawlikJeffrey E Gershenwald
Nov 3, 2006·Annals of Surgical Oncology·Stephan GretschelPeter M Schlag
Nov 30, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·R JungM Neumaier
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·E RacilaJ W Uhr
Jun 14, 2002·Seminars in Oncology·David N Krag, Donald L Weaver
Feb 26, 2008·Journal of Gastroenterology·Yoichi Ishizaki, Seiji Kawasaki
Jan 26, 2010·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·X ZhangP Lin
Nov 15, 2002·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·D C P CobbenH J Hoekstra
Jan 21, 2000·The Journal of Pathology·S A Burchill, P J Selby
Jul 8, 2009·Expert Review of Molecular Diagnostics·Luciane R Cavalli

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