Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections.

Journal of Clinical Microbiology
G E KennyH M Foy

Abstract

The sensitivities and specificities of isolation and serology for detection of Mycoplasma pneumoniae infections were determined for 3,546 pneumonia patients for whom both isolation and serological data were available. Soy peptone, fresh yeast extract, horse serum-supplemented agar, and diphasic medium were employed for isolation, and the lipid antigen of M. pneumoniae was used for serodiagnosis by complement fixation. The number of M. pneumoniae colonies most frequently detected was 200 to 600 per throat swab, with a range of less than or equal to 60 to greater than or equal to 2,000. The use of diphasic medium increased the number of isolates by 26% compared with direct isolation on agar plates. The organism was isolated from 360 of 525 patients who showed fourfold or greater antibody increases in their paired sera, resulting in a sensitivity of culture of 68%. When persons with titers of greater than or equal to 32 but no fourfold increase were used as the reference, the sensitivity of culture was 58%. The combined sensitivity of the culture method for persons with serological evidence of infection (fourfold increase and titer of greater than or equal to 32) was 64%. The specificity of the culture method was 97% for the 2,527...Continue Reading

References

Apr 1, 1979·The Journal of Infectious Diseases·J G TullyR P Wenzel
Jun 1, 1979·The Journal of Infectious Diseases·H M FoyI D Allan
Nov 1, 1976·American Journal of Epidemiology·E A EdwardsR O Peckinpaugh
Nov 1, 1989·Journal of Clinical Microbiology·C BernetJ Bonnet
Nov 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J S JensenK Lind
Apr 1, 1989·Clinical Microbiology Reviews·S A Morse
Apr 1, 1988·Diagnostic Microbiology and Infectious Disease·N CimolaiD E Woods
Jun 1, 1988·Diagnostic Microbiology and Infectious Disease·R C TiltonR W Ryan
Nov 1, 1988·Journal of Clinical Microbiology·J B BasemanD L Rose
Jul 28, 1967·Annals of the New York Academy of Sciences·G E Kenny
Jun 1, 1971·Journal of Bacteriology·E S Boatman, G E Kenny
Jul 28, 1967·Annals of the New York Academy of Sciences·R M ChanockL B Senterfit
May 26, 1966·The New England Journal of Medicine·T J Vecchio
Feb 1, 1983·American Journal of Clinical Pathology·S A DormanT F Smith
Jun 1, 1983·Journal of Clinical Microbiology·P A GranatoL B Weiner
May 1, 1980·American Journal of Clinical Pathology·P A GranatoL B Weiner
Aug 1, 1982·The Journal of Infectious Diseases·M Kleemola, H Käyhty
Oct 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·M E POLLOCKJ T SYVERTON
Jan 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·R M CHANOCKM F BARILE
Jul 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·R M CHANOCKB FORSYTH
Sep 20, 1965·JAMA : the Journal of the American Medical Association·J P GRIFFIN, Y E CRAWFORD

❮ Previous
Next ❯

Citations

Oct 19, 2004·Clinical Microbiology Reviews·Ken B Waites, Deborah F Talkington
Feb 17, 2005·Journal of Medical Microbiology·Riitta RätyMarjaana Kleemola
Feb 11, 2011·Current Infectious Disease Reports·Ari Bitnun, Susan E Richardson
Jun 22, 1995·The New England Journal of Medicine·K Asai, S Ohta
Dec 1, 1993·Journal of Clinical Pathology·M KarppelinA Miettinen
Feb 24, 2006·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·S TsiodrasH Giamarellou
Dec 20, 2005·Scandinavian Journal of Infectious Diseases·Jonas HedlundUNKNOWN Community-Acquired Pneumonia Working Group of the Swedish Society of Infectious Diseases
Dec 13, 2006·Pediatric Dermatology·Peter C SchalockJodi K Wenger
Aug 1, 1991·Epidemiology and Infection·K Lind, M W Bentzon
May 12, 2006·Journal of Medical Microbiology·Esaki Muthu ShankarUsha Anand Rao
May 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A BitnunS Richardson
Mar 5, 2016·Journal of Negative Results in Biomedicine·Mariana HerreraLázaro Agustín Vélez
Jun 19, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Samiah A Al-ZaidyAri Bitnun
Oct 25, 2020·NPJ Systems Biology and Applications·Erika GaspariMaria Suarez-Diez
Oct 1, 1994·Clinical Microbiology Reviews·V S Baselski, R G Wunderink
Feb 18, 1998·Journal of Clinical Microbiology·M Abele-HornJ Heesemann
Jul 26, 2003·Seminars in Pediatric Infectious Diseases·Ari BitnunSusan Richardson
May 1, 1996·Journal of Clinical Microbiology·T S AlexanderD H Willis
Feb 1, 1996·Journal of Clinical Microbiology·J S JensenK Lind
Dec 1, 1996·Journal of Clinical Microbiology·J W Dorigo-ZetsmaL Spanjaard
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F DaxboeckC Wenisch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.