Diagnosis of neuroblastoma and ganglioneuroma using Raman spectroscopy

Journal of Pediatric Surgery
Raja RabahMichael D Klein

Abstract

Raman spectroscopy has proven to be useful in studying premalignant and malignant lesions in adults. This is the first report to evaluate Raman spectroscopy in the diagnosis and classification of neuroblastoma in children. A biopsy or resection of fresh tissue samples from normal adrenal glands, neuroblastomas, ganglioneuromas, nerve sheath tumors, and pheochromocytoma at our hospital were equally divided between routine histology and spectroscopic studies. At least 12 spectra were collected from different regions of each sample using a Renishaw Raman microscope. Raw spectra were processed to remove noise, fluorescence, and shot noise, and then analyzed using principle component analysis and discriminant function analysis. We collected 698 spectra from 16 neuroblastomas, 5 ganglioneuromas, 3 normal adrenal glands, 6 nerve sheath tumors, and 1 pheochromocytoma. Raman spectroscopy differentiated between normal adrenal gland, and neuroblastoma and ganglioneuroma with 100% sensitivity and 100% specificity. It correlated well with the Shimada histologic classification system with 100% sensitivity and 100% specificity. It was also able to differentiate neuroblastoma from nerve sheath tumors and pheochromocytoma with high sensitivity ...Continue Reading

References

May 1, 1996·Endoscopy·A G Bohorfoush
Aug 22, 2000·The Journal of Investigative Dermatology·T R HataL K Pershing
Mar 3, 2004·Faraday Discussions·Nicholas StoneHugh Barr
Jun 9, 2004·Journal of Pediatric Surgery·Attila LorinczMichael D Klein
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Abigail S HakaMichael S Feld
Aug 10, 2006·Biopolymers·M V P ChowdaryC Murali Krishna

❮ Previous
Next ❯

Citations

Nov 13, 2012·Pediatric Surgery International·Alexander W AunerMichael D Klein
Nov 12, 2013·Talanta·Marko DakovićIvanka Holclajtner-Antunović
Nov 8, 2013·Lasers in Medical Science·José Luis González-SolísPascual Palomares-Anda
Feb 11, 2014·Cancer Metastasis Reviews·Rachel E KastGregory W Auner
Jul 14, 2010·Journal of Pediatric Surgery·Rachel KastMichael D Klein
Mar 13, 2010·Journal of Pediatric Surgery·Chad A Lieber, Mustafa H Kabeer
Jun 16, 2009·Journal of Pediatric Surgery·Hale WillsMichael D Klein
Apr 30, 2009·The International Journal of Medical Robotics + Computer Assisted Surgery : MRCAS·Brady W KingAbhilash K Pandya
Mar 25, 2010·The International Journal of Medical Robotics + Computer Assisted Surgery : MRCAS·Brady W KingAbhilash K Pandya
Jun 13, 2016·Lasers in Medical Science·Enrique Vargas-ObietaJosé Luis González-Solís
May 28, 2019·Analytical and Bioanalytical Chemistry·Olga GuselnikovaOleksiy Lyutakov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.