Diagnosis of occult hepatitis C without the need for a liver biopsy

Journal of Medical Virology
Inmaculada CastilloVicente Carreno

Abstract

The diagnosis of occult hepatitis C virus (HCV) infection is based on the presence of HCV-RNA in the liver. This study aimed to evaluate the use of combining non-invasive assays to diagnose occult HCV. A total of 122 patients with occult HCV (HCV-RNA in the liver without detectable anti-HCV and serum HCV-RNA) and 45 patients with cryptogenic chronic hepatitis (without HCV-RNA in the liver and negative for anti-HCV and serum HCV-RNA) were included. HCV-RNA was tested in peripheral blood mononuclear cells (PBMCs) and in 2 ml of ultracentrifuged serum. Anti-core HCV was examined by a non-commercial enzyme-linked immunosorbent assay. All controls were negative for the three HCV markers studied. Among patients with occult HCV, 36% were anti-core HCV positive, 57% had serum HCV-RNA after ultracentrifugation, and 61% had HCV-RNA in PBMCs. Combining the results of the assays, 91% of the patients were positive for at least one marker. Intrahepatic HCV-RNA load was significantly higher in patients who were positive simultaneously for the three HCV markers than in patients who were negative for all markers (P = 0.006) and than in those with one or two HCV markers (P = 0.039). Replication of HCV in liver was detected more frequently in pat...Continue Reading

References

May 18, 1989·Nature·S Kwok, R Higuchi
Oct 21, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vicente CarreñoMargarita Pardo
Mar 25, 2008·Journal of Medical Virology·Satawat ThongsawatKenrad E Nelson
Aug 8, 2008·Journal of the American Society of Nephrology : JASN·Guillermina BarrilVicente Carreño
Nov 21, 2008·Journal of the American Society of Nephrology : JASN·Fabrizio Fabrizi, Paul Martin
May 29, 2009·Journal of Medical Virology·Inmaculada CastilloVicente Carreño
Jan 15, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Tram N Q PhamTomasz I Michalak
Apr 13, 2010·Immunological Investigations·Hosam Zaghloul, Walid El-Sherbiny

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Citations

Nov 3, 2010·Virology Journal·Saira MunirZunaira Awan
Jun 28, 2012·World Journal of Gastroenterology : WJG·Vicente CarreñoJuan Antonio Quiroga
Oct 23, 2014·Hepatitis Monthly·Mohammad Saeid Rezaee ZavarehSeyed Yasser Saiedi Hosseini
Oct 12, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vicente Carreño
Feb 18, 2011·Journal of Gastroenterology·Angela Dolganiuc, Gyongyi Szabo
Apr 7, 2011·Enfermedades infecciosas y microbiología clínica·Vicente Carreño GarcíaJuan Antonio Quiroga Estévez
Oct 15, 2011·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·David J PinatoMario Pirisi
Dec 6, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mohammad Saeid Rezaee-ZavarehSeyed Moayed Alavian
Oct 17, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·María C Roque-CuéllarJosé Aguilar-Reina
Jun 5, 2014·Journal of Medical Virology·Pavlina Dzekova VidimliskiAleksandar Sikole
Jul 30, 2015·Gastroenterology Research and Practice·Bashar M Attar, David Van Thiel
Dec 28, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Muhammad IdreesFayyaz Ahmed Malik
Feb 6, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A K Le PageW D Rawlinson
Sep 26, 2016·Archives of Virology·Ghada Fahmy HelalyAbd El-Gaffar Sabry Oluyemi
Jul 22, 2019·Journal of Viral Hepatitis·Aldo MarroneMarc Ghany
Jan 7, 2016·Iranian Red Crescent Medical Journal·Mohammad Saeid Rezaee-ZavarehSeyed Moayed Alavian
Dec 12, 2017·Journal of Clinical and Translational Hepatology·Mohamed Darwish Ahmed Abd AllaMostafa Kamel El-Awady
Sep 27, 2019·Viral Immunology·Aleena HaqqiMuhammad Sohail Afzal
Jun 29, 2018·Journal of Clinical and Translational Hepatology·Mohamed Darwish Ahmed Abd AllaMostafa Kamel El Awady
Jun 29, 2018·Journal of Clinical and Translational Hepatology·Alyssa Austria, George Y Wu
Aug 26, 2020·Expert Review of Clinical Pharmacology·Kimia KardaniAzam Bolhassani
Jun 9, 2020·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Shahab MahmoudvandSheida B Sheikhrobat
Oct 14, 2020·International Journal of Immunopathology and Pharmacology·Abeya A LotfiAhmed S Abdel-Moneim
Jan 19, 2021·Transfusion·Timothy AnHelen M Faddy
Apr 7, 2021·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Arwa KamhawyGamal Esmat

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