Diagnosis of prostate cancer by detection of minichromosome maintenance 5 protein in urine sediments.

British Journal of Cancer
Tim J DudderidgeK Stoeber

Abstract

The accuracy of prostate-specific antigen (PSA) testing in prostate cancer detection is constrained by low sensitivity and specificity. Dysregulated expression of minichromosome maintenance (Mcm) 2-7 proteins is an early event in epithelial multistep carcinogenesis and thus MCM proteins represent powerful cancer diagnostic markers. In this study we investigate Mcm5 as a urinary biomarker for prostate cancer detection. Urine was obtained from 88 men with prostate cancer and from two control groups negative for malignancy. A strictly normal cohort included 28 men with complete, normal investigations, no urinary calculi and serum PSA <2 ng ml(-1). An expanded control cohort comprised 331 men with a benign final diagnosis, regardless of PSA level. Urine was collected before and after prostate massage in the cancer patient cohort. An immunofluorometric assay was used to measure Mcm5 levels in urine sediments. The Mcm5 test detected prostate cancer with 82% sensitivity (confidence interval (CI)= 72-89%) and with a specificity ranging from 73 (CI=68-78%) to 93% (CI=76-99%). Prostate massage led to increased Mcm5 signals compared with pre-massage samples (median 3440 (interquartile range (IQR) 2280 to 5220) vs 2360 (IQR <1800 to 4360);...Continue Reading

Associated Clinical Trials

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Citations

Nov 12, 2014·Molecular Biology International·Mitali DasGopeshwar Narayan
May 30, 2012·Oncology Reports·Sarah K MartinNatasha Kyprianou
Aug 25, 2011·Biomarkers in Medicine·Mark R Haythorn, Richard J Ablin
Oct 23, 2015·Expert Review of Molecular Diagnostics·Maria FrantziHarald Mischak
Oct 13, 2011·The Journal of Pathology·Gareth H Williams, Kai Stoeber
Apr 14, 2018·Frontiers in Molecular Biosciences·Yeon-Soo Seo, Young-Hoon Kang
Aug 22, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Yifei WangWei Kang
Dec 19, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dan WangHongyan Wang

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

SPSS
Multicalc Advanced Immunoassay Data Management

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