Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation.

Journal of Clinical Microbiology
G A DenoyelD Peyramond

Abstract

A solid-phase reverse immunosorbent test (SPRIST) based on the addition of an excess of rubella virus hemagglutinin was evaluated for the demonstration of rubella-specific immunoglobulin M (IgM), and the results were compared with those of the density gradient centrifugation technique. In a retrospective study in which 157 sera were tested, the two techniques yielded identical results (55 IgM-positive and 102 IgM-negative samples). In a prospective study, 592 sere were examined; 8 IgM-positive results by SPRIST corresponded to a recent rubella infection or vaccination. Neither rheumatoid factor nor heterophil antibody seemed to interfere with the results of SPRIST. This test would be a useful and rapid routine technique for demonstration of the presence of virus-specific IgM in serum samples, particularly for viruses with a hemagglutinin. Except for anti-human IgM, no more reagents are needed than for widely used hemagglutination inhibition procedures.

References

Jan 1, 1979·Journal of Medical Virology·W DuermeyerJ van der Veen
Jan 1, 1973·Annals of the Rheumatic Diseases·P V ShirodariaF Stanford
Mar 9, 1967·The New England Journal of Medicine·G L StewartH M Meyer

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Citations

Jan 1, 1989·Critical Reviews in Clinical Laboratory Sciences·F V PlappJ M Rachel
May 1, 1984·Journal of Clinical Pathology·J Hodgson, P Morgan-Capner
May 1, 1989·Journal of Medical Virology·G DuverlieJ Orfila
Jan 1, 1982·Journal of Medical Virology·J T van der LogtJ van der Veen
Jan 2, 1988·British Medical Journal·P LebonM Arthuis
Jan 1, 1985·Journal of Clinical Microbiology·G A Linde

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