PMID: 7027594May 16, 1981Paper

Diagnosis of respiratory syncytial virus infection in the bovine respiratory tract by immunofluorescence

The Veterinary Record
L H Thomas, E J Stott

Abstract

Direct staining of nasopharyngeal smears with hyperimmune bovine serum raised against respiratory syncytial virus (RSV) and conjugated with fluorescein isothyocyanate was compared with virus isolation from 14 experimentally infected animals. Twenty-four out of 107 specimens examined were positive by the fluorescent antibody (FA) method and 21 out of 107 by virus isolation. Most of the FA positives (81 per cent) were detected after the ninth day of infection whereas 80 per cent of virus isolations were made before this time. Only one false positive (one out of 57 specimens) was detected by the FA method in nine uninfected control animals. Non-specific fluorescence presented some problems when examining nasopharyngeal material. The same conjugate was found to be more useful in detecting RSV antigen in lung tissue of eight experimentally infected animals and 11 out of 22 naturally occurring cases of calf pneumonia from five outbreaks of disease. In three of the outbreaks the diagnosis was confirmed by virus isolation and serology. The dominating histopathological response in both the experimental and the natural disease was an acute bronchiolitis and alveolitis. The finding of RSV antigen in association with these lesions provides...Continue Reading

Citations

Jan 1, 1990·Archives of Virology·T G Kimman, F Westenbrink
Mar 1, 1989·Veterinary Immunology and Immunopathology·R S Stewart, L J Gershwin
Jun 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A J MasotE Redondo
Feb 1, 1996·Australian Veterinary Journal·P R ScottB G Lowman
Jul 14, 2010·The Veterinary Clinics of North America. Food Animal Practice·Bruce W Brodersen
Apr 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E B BelknapJ C Baker
Nov 29, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N Z ElerakyL N Potgieter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
F A OsorioD Grotelueschen
Reproduction in Domestic Animals = Zuchthygiene
K AlmM Andersson
© 2021 Meta ULC. All rights reserved