Diagnosis of thalassemia on dried blood spot samples by high performance liquid chromatography

Hemoglobin
Sakorn PornprasertKanyakan Sukunthamala

Abstract

High performance liquid chromatography (HPLC) on fresh lysates is the standard test for identification of thalassemia. Samples in the form of dried blood spot(s) (DBS) mailed to reference laboratories where HPLC is available could be an alternative. Hemoglobin (Hb) on DBS at day 1, 7, 15 and 30 were analyzed by HPLC and compared to those analyzed from fresh liquid whole blood at day 0. A 100% consistent interpretation of β-thalassemia (β-thal) trait and β-thal/Hb E disease between liquid whole blood and DBS was observed when analyzing Hb A(2) on DBS at a level of 2.7-9.9% in conjunction with a lower MCV (<80 fL) and MCH (<27 pg) and analyzing β-thal/Hb E by using Hb E and Hb F at a level of 30-48% and >10%, respectively. Therefore, our results show that detection of thalassemia carriers using DBS is possible and is the alternative of choice in a low resource setting.

References

Jan 1, 1976·American Journal of Hematology·P T Rowley
Jul 1, 1997·Hemoglobin·Supan Fucharoen, P Winichagoon
Aug 25, 2004·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S SirichotiyakulT Tongsong
Jun 9, 2007·Clinical Chemistry·Yvonne A DanielR Neil Dalton
Aug 19, 2007·Indian Journal of Pediatrics·R B ColahA C Gorakshakar
Jun 3, 2009·International Journal of Laboratory Hematology·P Van DelftP C Giordano

Citations

Aug 16, 2013·Hemoglobin·Sakorn Pornprasert, Siratcha Phanthong
Oct 9, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Domenico Dell'EderaGiancarlo Di Renzo
Nov 11, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn PornprasertKallayanee Treesuwan

Related Concepts

Collection of Blood Specimen for Laboratory Procedure
High Pressure Liquid Chromatography Procedure
Fetal Hemoglobin
Hemoglobin A2
Hemoglobin E
Eryhem
Hemolysis
Thalassemia
High Pressure Liquid Chromatography Procedure
Liquid Chromatography

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.