Diagnosis of toxoplasmosis by joint detection of immunoglobulin A and immunoglobulin M.

Journal of Clinical Microbiology
M ArcaviG Griemberg

Abstract

An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the results obtained were compared with those obtained by methods routinely used for the detection of acute-phase markers, IgA and IgM IIF and enzyme immunoassay. Accordingly, 114 serum samples were selected from patients showing titers of > or = 1:1,024 by IgG,A,M-IIF. (i) In 90 of the samples, neither IgA nor IgM was detected by any of the methods employed; (ii) the remaining 24 samples showed IgA and/or IgM. In all cases, the IgG,A,M-IIF assay with absorbed sera was positive. These comparative data support the use of IgG,A,M-IIF, performed with absorbed and unabsorbed sera simultaneously, for determining the presence of specific IgG, IgA, and IgM by employing a single technique (IIF), one conjugate (anti-IgG,A,M), and only one sample (with and without previous absorption), thus providing a useful initial tool for the diagnosis of toxoplasmosis.

References

Feb 1, 1992·Clinical and Experimental Immunology·A DecosterM Delcroix
Feb 1, 1991·Pediatrics in Review·B J Freij, J L Sever
Jul 1, 1990·The Journal of Infectious Diseases·P Stepick-BiekJ S Remington
Dec 1, 1990·Journal of Clinical Microbiology·J HuskinsonJ S Remington
Mar 2, 1985·Lancet·G DesmontsM Chartier
Mar 1, 1988·The Journal of Infection·R E Holliman
Sep 1, 1987·Reviews of Infectious Diseases·R G BrooksJ S Remington
Dec 1, 1987·Journal of Clinical Microbiology·M WilsonK W Walls
Feb 4, 1988·The New England Journal of Medicine·R McCabe, J S Remington
Aug 1, 1985·Pediatric Clinics of North America·J K Frenkel
Jun 1, 1983·Journal of Clinical Microbiology·F WielaardE H van Elven
Aug 1, 1980·The Journal of Infectious Diseases·P C WelchJ S Remington
May 1, 1981·Journal of Clinical Microbiology·E L FrancoA J Sulzer
May 1, 1982·The Journal of Infectious Diseases·Y NaotJ S Remington
Jul 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F FoudrinierJ M Pinon
Apr 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J G Montoya, J S Remington
Oct 1, 1994·Pediatric Clinics of North America·J K Stamos, A H Rowley
Jun 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S Y Wong, J S Remington
Aug 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·U GrossJ Heesemann
Jul 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J M Francis, D H Joynson
Mar 1, 1993·The Journal of Infectious Diseases·M LappalainenK Hedman
Mar 1, 1993·Baillière's Clinical Obstetrics and Gynaecology·B Stray-Pedersen

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