Diagnostic accuracy of loop-mediated isothermal amplification as a near-patient test for meningococcal disease in children: an observational cohort study

The Lancet Infectious Diseases
Thomas W BourkeDerek J Fairley

Abstract

Diagnosis of meningococcal disease relies on recognition of clinical signs and symptoms that are notoriously non-specific, variable, and often absent in the early stages of the disease. Loop-mediated isothermal amplification (LAMP) has previously been shown to be fast and effective for the molecular detection of meningococcal DNA in clinical specimens. We aimed to assess the diagnostic accuracy of meningococcal LAMP as a near-patient test in the emergency department. For this observational cohort study of diagnostic accuracy, children aged 0-13 years presenting to the emergency department of the Royal Belfast Hospital for Sick Children (Belfast, UK) with suspected meningococcal disease were eligible for inclusion. Patients underwent a standard meningococcal pack of investigations testing for meningococcal disease. Respiratory (nasopharyngeal swab) and blood specimens were collected from patients and tested with near-patient meningococcal LAMP and the results were compared with those obtained by reference laboratory tests (culture and PCR of blood and cerebrospinal fluid). Between Nov 1, 2009, and Jan 31, 2012, 161 eligible children presenting at the hospital underwent the meningococcal pack of investigations and were tested for...Continue Reading

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Citations

Oct 13, 2015·The Pediatric Infectious Disease Journal·Thomas Walter BourkeMichael David Shields
Mar 3, 2015·The Lancet Infectious Diseases·Nathan C Bahr, David R Boulware
Feb 11, 2016·The Pediatric Infectious Disease Journal·Thomas W BourkeMichael D Shields
Jun 7, 2016·Lancet·Fiona McGillTom Solomon
Jan 25, 2017·Current Opinion in Infectious Diseases·Fiona McGillTom Solomon
Jan 1, 2017·Journal of the Royal Army Medical Corps·Beverley C MillarJ E Moore
Nov 20, 2016·Journal of Clinical Pathology·Angel BoulosPeter Coyle
Mar 9, 2018·Archives of Disease in Childhood. Education and Practice Edition·Thomas WaterfieldMark D Lyttle
Mar 30, 2018·Frontiers in Pediatrics·Mitsuko SekiDong Wook Kim
Jun 27, 2020·Archives of Disease in Childhood·Thomas WaterfieldUNKNOWN Paediatric Emergency Research in the UK and Ireland (PERUKI)

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