Diagnostic accuracy of PCR for Jaagsiekte sheep retrovirus using field data from 125 Scottish sheep flocks

The Veterinary Journal
F I LewisG J Gunn

Abstract

Using a representative sample of Scottish sheep comprising 125 flocks, the sensitivity and specificity of PCR for Jaagsiekte sheep retrovirus (JSRV) was estimated. By combining and adapting existing methods, the characteristics of the diagnostic test were estimated (in the absence of a gold standard reference) using repeated laboratory replicates. As the results of replicates within the same animal cannot be considered to be independent, the performance of the PCR was calculated at individual replicate level. The median diagnostic specificity of the PCR when applied to individual animals drawn from the Scottish flock was estimated to be 0.997 (95% confidence interval [CI] 0.996-0.999), whereas the median sensitivity was 0.107 (95% CI 0.077-0.152). Considering the diagnostic test as three replicates where a positive result on any one or more replicates results in a positive test, the median sensitivity increased to 0.279. Reasons for the low observed sensitivity were explored by comparing the performance of the test as a function of the concentration of target DNA using spiked positive controls with known concentrations of target DNA. The median sensitivity of the test when used with positive samples with a mean concentration of...Continue Reading

References

May 10, 2000·Preventive Veterinary Medicine·M Greiner, I A Gardner
Dec 8, 2004·Preventive Veterinary Medicine·A J BranscumW O Johnson
Jul 18, 2006·Annals of Epidemiology·Haitao ChuSander Greenland

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Citations

Dec 29, 2012·Emerging Themes in Epidemiology·Fraser I Lewis, Paul R Torgerson
Jun 30, 2011·The Journal of Veterinary Medical Science·Naoyoshi MaedaToshimitsu Uede
Jul 17, 2014·Veterinary Medicine International·Sandip ChakrabortyMinakshi Prasad
May 21, 2019·Frontiers in Oncology·Mark E GrayDavid Argyle
Feb 7, 2021·Animals : an Open Access Journal From MDPI·Marcelo De Las HerasAurora Ortín

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