Diagnostic and prognostic value of bladder cancer-related transcript markers in urine

Journal of Cancer Research and Clinical Oncology
Juliane SchmidtSusanne Fuessel

Abstract

Since cytology as the current "gold standard" for noninvasive detection of bladder cancer (BCa) is characterized by a relatively low sensitivity, urinary transcript levels of survivin (SVV), Ki-67 and cytokeratin 20 (CK20) were evaluated as alternative or complementary biomarkers. Furthermore, their prognostic value was investigated. Voided urine samples from 105 BCa patients and 156 controls were included. Total RNA was isolated from urine pellets and reverse-transcribed into cDNA. Expression levels of SVV, Ki-67 and CK20 were determined by quantitative PCR and normalized to the housekeeping gene TBP. Diagnostic performance of transcript markers and cytology was assessed by receiver operating characteristic (ROC) curve analyses. The prognostic value of the transcript markers was calculated by Cox proportional hazards models. ROC analyses resulted in AUC values between 0.71 (Ki-67) and 0.86 (CK20), indicating an appropriate diagnostic power. Using specifically defined cutoff values, the expression levels of the assessed biomarkers were significantly higher in urine specimens from BCa patients compared to control group (Mann-Whitney U test p < 0.001). Specificity ranged from 75% (SVV) to 84% (CK20) and sensitivity from 56% (Ki-6...Continue Reading

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Citations

May 1, 2018·Expert Review of Molecular Diagnostics·Moritz MaasTilman Todenhöfer
Jun 23, 2018·Asia-Pacific Journal of Clinical Oncology·Yan MiXin Liu
May 10, 2017·Journal of Cancer Research and Clinical Oncology·Karsten SalomoSusanne Fuessel

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