Diagnostic application of Epstein-Barr virus-encoded RNA in situ hybridisation

Pathology
Maurice LoughreyPaul Waring

Abstract

Epstein-Barr virus (EBV) has been implicated in the pathogenesis of nasopharyngeal carcinoma and a range of proliferative lymphoid conditions. In situ hybridisation (ISH) looking for virus-encoded RNA (EBER) transcripts is performed simply using a commercially available probe. We aimed to examine the application of this test in a routine diagnostic setting. In total, 26 cases in which EBV ISH was requested for diagnostic purposes were examined. We looked at the indication for testing, the result and its implication for the final diagnosis. Cases were classified into three categories: possible nasopharyngeal carcinoma; possible EBV-related lymphoma; and possible immunodeficiency-associated lymphoproliferative disorder. Six of nine cases of possible nasopharyngeal carcinoma were EBV ISH positive (3/3 primary and 3/6 secondary), confirming the diagnosis. Three of 14 possible lymphoma cases were EBV ISH positive which, along with appropriate ancillary tests, assisted in making the diagnoses of Burkitt's lymphoma, lymphomatoid granulomatosis and extranodal NK/T-cell lymphoma of nasal type. All of three immunodeficiency-associated cases were EBV ISH positive. Two of these were post-transplant lymphoproliferative disorders, monomorphi...Continue Reading

Citations

Apr 30, 2008·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Naim K FanaianBahig M Shehata
Sep 16, 2008·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Jean-Louis DargentAnne Françoise Gennotte
Jun 8, 2007·Journal of the International Association of Physicians in AIDS Care : JIAPAC·Roberto ManfrediGinevra Marinacci
Sep 17, 2018·BMJ Case Reports·Christian N FranciscoVida Margarette de Vera Andal
Aug 12, 2017·International Journal of Dentistry·Luana L MartinsKarem L Ortega

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