Diagnostic applications of haemopoietic progenitor culture techniques in polycythaemias and thrombocythaemias

Leukemia & Lymphoma
Nigel B Westwood, T C Pearson

Abstract

Clonogenic cultures support the proliferation of haemopoietic cells into colonies of differentiated progeny. Using these techniques it has been established that normal haemopoietic progenitors are dependent upon growth factors for their survival, proliferation and differentiation in vitro. However, progenitors from patients with polycythaemia vera (PV) generate erythroid colonies in cultures deprived of exogenous erythropoietin. These have been termed endogenous erythroid colonies (EEC). Recently, endogenous megakaryocytic colonies (EMC), those arising in assays without megakaryocyte growth factors, have been reported, particularly in patients with primary thrombocythaemia (PT). Many investigators have found an EEC positive culture to have 100% diagnostic specificity and sensitivity for PV. Similarly, EMC have been shown by some to be an unequivocal marker of PT. Accordingly, clonogenic assays for EEC and EMC have been advocated as diagnostic markers of PV and PT. However, the specificity of these assays is not universally attested to as there are some reports of EEC in patients with secondary polycythaemia and of EEC and EMC in normal subjects. Thus, for diagnostic use, EEC and EMC assays must be exhaustively validated for spe...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G R Johnson, D Metcalf
May 1, 1977·The Journal of Clinical Investigation·E D ZanjaniL R Wasserman
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D MetcalfB Sordat
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J R StephensonM M Shreeve
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Jan 1, 1973·The International Journal of Social Psychiatry·D A Rockwell
Jun 13, 1974·The New England Journal of Medicine·R Gabriel
Jun 13, 1974·The New England Journal of Medicine·J F Prchal, A A Axelrad
Oct 1, 1971·British Journal of Haematology·L R Wasserman
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·Y IchikawaL Sachs
Aug 1, 1970·Journal of Cellular Physiology·B L Pike, W A Robinson
Apr 1, 1983·American Journal of Clinical Pathology·B S BeckmanJ W Fisher
Jul 1, 1982·British Journal of Haematology·J Mladenovic, J W Adamson
Feb 1, 1980·British Journal of Haematology·C LacombeB Varet
Sep 1, 1993·Blood Reviews·M Y Gordon

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Citations

Apr 2, 2005·Annals of Hematology·Juergen ThieleVolker Diehl
May 30, 2001·Experimental Hematology·S RöderH L Pahl
Jan 20, 2000·Baillière's Clinical Haematology·R Kralovics, J T Prchal
Jun 13, 2002·British Journal of Haematology·Claire N Harrison
Jun 10, 2000·European Journal of Biochemistry·H L Pahl
Mar 24, 1999·Current Opinion in Hematology·J F Prchal, J T Prchal
Mar 23, 2002·Leukemia & Lymphoma·Ayalew Tefferi
Apr 28, 2000·Leukemia & Lymphoma·M GriesshammerH Schrezenmeier
Dec 7, 2007·Ophthalmology·Emmanuel HéronOlivier Hermine
Apr 2, 2005·British Journal of Haematology·Philipp S GoerttlerHeike L Pahl
May 27, 2009·British Journal of Haematology·Manuel MutschlerHeike L Pahl
Sep 1, 2000·The American Journal of Medicine·A TefferiM N Silverstein
Mar 31, 1999·Baillière's Clinical Haematology·A E von dem BorneM de Haas
Jun 20, 2006·Best Practice & Research. Clinical Haematology·Claire N Harrison, Anthony R Green
Oct 8, 2005·Seminars in Hematology·Anthony J Bench, Heike L Pahl
May 25, 2002·British Journal of Haematology·Caroline ZwickyAnoreas Tobler
Feb 1, 1997·Baillière's Clinical Haematology·J BriereE Peynaud-Debayle
Jan 16, 2004·Journal of Occupational and Environmental Medicine·Miriam QuittPaul Froom
Dec 21, 2007·The Korean journal of laboratory medicine·Jeong Yeal AhnJae Hoon Lee
Jul 21, 2005·British Journal of Haematology·Claire N Harrison
Apr 1, 2004·Clinical and Laboratory Haematology·S-K TeyA Mills
Oct 6, 1997·European Journal of Haematology·B AndréassonJ Kutti
Aug 2, 2006·Archives of Pathology & Laboratory Medicine·Ming CaoYouli Zu
Jan 20, 2000·Baillière's Clinical Haematology·T C Pearson

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