Abstract
Kaposi's sarcoma (KS), caused by HHV-8, is the most frequent HIV-associated malignancy worldwide and remains a major scourge in Sub-Saharan Africa. KS is also endemic in much of Africa. There is a risk of misdiagnosis based solely on clinical appearance and haematoxylin and eosin (H&E) staining, especially with other reactive and neoplastic vascular proliferations which occur in the mouth. This study examined oral and cutaneous biopsies from clinically diagnosed lesions of KS in Kenya, using histopathology supplemented with immunohistochemistry (IHC) and polymerase chain reaction (PCR) for HHV-8 as confirmation of diagnosis. Biopsies of 49 lesions (28 oral, 21 cutaneous) previously diagnosed as 'KS' were re-examined by H&E staining and IHC targeting HHV-8 LANA-1. Positive controls were sections from embedded BCBL-1 cell lines. Negative controls were from three different HHV-8-negative biopsies. Confirmation of HHV-8 immunohistochemistry was sought by PCR and by determining the HHV-8 ORFK1 subtype. Whilst most cases were confirmed, 12 oral and 4 cutaneous lesions displayed clinical and histological features of KS but were negative to HHV-8 IHC. These oral lesions were re-diagnosed as pyogenic granulomata (n = 6), deep mycosis (n...Continue Reading
References
Aug 1, 1983·The Journal of Dermatologic Surgery and Oncology·A E Friedman-Kien
Dec 9, 2000·Virology·V LacosteA Gessain
Jun 14, 2002·The Lancet Infectious Diseases·Ulrich R HenggeSiegfried Seeber
Apr 10, 2003·Medical Microbiology and Immunology·Peter A Reichart
May 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Bhavna H ChohanRhoda Ashley Morrow
Jan 11, 2007·American Journal of Clinical Pathology·David A WadaScott R Florell
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 14, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Fatemeh PakPeter Biberfeld
Feb 1, 2008·Cancer Letters·Martin M NsubugaSam M Mbulaiteye
Apr 9, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tiffany WhiteThomas B Campbell
Jul 29, 2008·Diagnostic Pathology·Wayne Grayson, Liron Pantanowitz
Sep 12, 2008·International Journal of Oral and Maxillofacial Surgery·F M A ButtF G Machigo
Jan 22, 2009·PloS One·Christine JohnstonCorey Casper
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Sheila C DollardJeffrey N Martin
Sep 12, 2012·BMC Infectious Diseases·David J Speicher, Newell W Johnson
Citations
Apr 26, 2016·Oral Diseases·L T VernonN W Johnson
Jul 15, 2017·The Journal of Craniofacial Surgery·Paolo Garzino-DemoGuglielmo Ramieri
Apr 26, 2016·Oral Diseases·D J SpeicherA-B Moscicki
Mar 23, 2017·American Journal of Clinical Dermatology·Johann W Schneider, Dirk P Dittmer
May 16, 2019·Periodontology 2000·Stephen PorterStefano Fedele
May 16, 2019·Periodontology 2000·Kannan Ranganathan, Krishna Mohan Rao Umadevi