Diagnostic efficacy of real-time PCR for ocular cytomegalovirus infections

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Dai MiyazakiReal-time PCR for ocular cytomegalovirus infection study group

Abstract

The aim of this study is to determine the efficacy of quantitative real-time PCR (qPCR) and clinical characteristics to diagnose ocular cytomegalovirus (CMV) infections. The technical factors were assessed by the outcomes of the qPCR assay at five institutions in Japan using the WHO International Standard of cytomegalovirus. The clinical factors were assessed by examining the aqueous humor samples of 197 eyes of 197 consecutive patients suspected of CMV using the receiver operating characteristics (ROCs). All of the institutions had excellent detection efficacy, although the copy number ranged from 0.82 to 4.66 copies/IU. In the clinical samples, CMV was detected in 51 eyes, and the amount of CMV DNA was significantly higher for CMV retinitis. In corneal diseases, the amount of CMV DNA was significantly associated with frequency of recurrences and IOP elevations. The sensitivity and specificity of qPCR for the diagnosis was 90.0 and 98.7%, respectively. For the corneal and anterior uveitis types of CMV diseases, the area under the curve (AUC) of qPCR was 0.95 and 0.96, followed by frequency of recurrences with AUC of 0.89 and 0.82, and IOP elevations with AUC of 0.78 and 0.76. Unclassified cytomegalovirus detection, which did n...Continue Reading

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Citations

Oct 26, 2018·Reviews in Medical Virology·Paul D Griffiths
Aug 15, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Daisuke ShimizuYoshitsugu Inoue
Nov 8, 2019·Ocular Immunology and Inflammation·Chansathya KhieuAniki Rothova
Aug 23, 2020·Indian Journal of Ophthalmology·Kalpana BabuManfred Zierhut
Jan 5, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jason M L MillerHakan Demirci
Jan 15, 2021·Eye and Vision·Angela H Y WongKelvin H Wan
Jun 3, 2021·International Journal of Molecular Sciences·Dai MiyazakiSatoru Yamagami
Jul 27, 2021·American Journal of Ophthalmology Case Reports·Chia-Chen KaoShwu-Jiuan Sheu

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Methods Mentioned

BETA
PCR
Nucleic Acid Amplification

Software Mentioned

Stata

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