Diagnostic microchip to assay 3D colony-growth potential of captured circulating tumor cells

Lab on a Chip
Colette A BichselMatthias P Lutolf

Abstract

Microfluidic technology has been successfully applied to isolate very rare tumor-derived epithelial cells (circulating tumor cells, CTCs) from blood with relatively high yield and purity, opening up exciting prospects for early detection of cancer. However, a major limitation of state-of-the-art CTC-chips is their inability to characterize the behavior and function of captured CTCs, for example to obtain information on proliferative and invasive properties or, ultimately, tumor re-initiating potential. Although CTCs can be efficiently immunostained with markers reporting phenotype or fate (e.g. apoptosis, proliferation), it has not yet been possible to reliably grow captured CTCs over long periods of time and at single cell level. It is challenging to remove CTCs from a microchip after capture, therefore such analyses should ideally be performed directly on-chip. To address this challenge, we merged CTC capture with three-dimensional (3D) tumor cell culture on the same microfluidic platform. PC3 prostate cancer cells were isolated from spiked blood on a transparent PDMS CTC-chip, encapsulated on-chip in a biomimetic hydrogel matrix (QGel™) that was formed in situ, and their clonal 3D spheroid growth potential was assessed by mi...Continue Reading

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Citations

May 7, 2013·Annual Review of Biomedical Engineering·David W InfangerClaudia Fischbach
Apr 30, 2013·Lab on a Chip·Sunil K AryaAbdur Rub Abdur Rahman
Jun 4, 2013·Lab on a Chip·Nasim AnnabiAli Khademhosseini
Jul 28, 2015·International Journal of Molecular Sciences·Shih-Jie Lo, Da-Jeng Yao
Jun 28, 2014·Advanced Drug Delivery Reviews·Daniela LoessnerJudith Ann Clements
Dec 18, 2015·Acta Biomaterialia·Bumjin NamkoongUtkan Demirci
May 27, 2015·The Analyst·Parthiv Kant ChaudhuriChwee Teck Lim
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Jun 6, 2015·Biomicrofluidics·Shannon L FaleyLeon M Bellan
Mar 21, 2015·Biomedical Microdevices·Yoon-Tae KangYoung-Ho Cho
Jul 11, 2018·Biotechnology and Bioengineering·Sultan KhetaniAmir Sanati Nezhad
Dec 7, 2013·Cancers·Brian HuAmir Goldkorn
Mar 31, 2018·The Cancer Journal·Ashutosh AgarwalRichard J Cote
Oct 11, 2017·Nature Biomedical Engineering·Brian A AguadoLonnie D Shea
Aug 10, 2017·Oncology Letters·Weiwei LiuZhihua Tao

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